Alwash Mourouge Saadi, Aqma Wan Syaidatul, Ahmad Wan Yaacob, Ibrahim Nazlina
Department of Biology, College of Science, University of Babylon, Hillah, Iraq.
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Int J Microbiol. 2020 Feb 5;2020:6915483. doi: 10.1155/2020/6915483. eCollection 2020.
is one of the main causes of nosocomial infections and is frequently associated with opportunistic infections among hospitalized patients. Kaempferol-3--(2',6'-di--coumaroyl)--D glucopyranoside ( ) is an antipseudomonal compound isolated from the leaves of the native medicinal plant . Herein, an RNA-seq transcriptomic approach was employed to study the effect of treatment on and to elucidate the molecular mechanisms underlying the response to at two time points (6 h and 24 h incubation). Quantitative real-time PCR (qRT-PCR) was performed for four genes (, , , and ) to assess the reliability of the RNA-seq results. The RNA-seq transcriptomic analysis revealed that increases the expression of genes involved in the electron transport chain (NADH-I), resulting in the induction of ATP synthesis. Furthermore, also increased the expression of genes associated with ATP-binding cassette transporters, flagella, type III secretion system proteins, and DNA replication and repair, which may further influence nutrient uptake, motility, and growth. The results also revealed that decreased the expression of a broad range of virulence factors associated with LPS biosynthesis, iron homeostasis, cytotoxic pigment pyocyanin production, and motility and adhesion that are representative of an acute infection profile. In addition, pathways for amino acid synthesis and membrane lipid composition were modified to adapt to treatment. Overall, the present research provides a detailed view of adaptation and behaviour in response to and highlights the possible therapeutic approach of using plants to combat infections.
是医院感染的主要原因之一，并且经常与住院患者的机会性感染相关。山奈酚-3-O-(2',6'-二-O-香豆酰基)-D-吡喃葡萄糖苷（ ）是从本地药用植物的叶子中分离出的一种抗铜绿假单胞菌化合物。在此，采用RNA测序转录组学方法研究 处理对 的影响，并阐明在两个时间点（孵育6小时和24小时）对 反应的分子机制。对四个基因（ 、 、 和 ）进行定量实时PCR（qRT-PCR）以评估RNA测序结果的可靠性。RNA测序转录组分析表明， 增加了参与电子传递链（NADH-I）的基因的表达，导致ATP合成的诱导。此外， 还增加了与ATP结合盒转运蛋白、鞭毛、III型分泌系统蛋白以及DNA复制和修复相关的基因的表达，这可能进一步影响营养物质摄取、运动性和生长。结果还表明， 降低了与LPS生物合成、铁稳态、细胞毒性色素绿脓菌素产生以及运动性和粘附相关的多种毒力因子的表达，这些毒力因子代表急性 感染特征。此外，氨基酸合成和膜脂组成的途径被修饰以适应 处理。总体而言，本研究提供了 对 适应和行为的详细视图，并突出了使用植物对抗 感染的可能治疗方法。
