Oh Se-Joon, Choi Kyung-Un, Choi Sung-Won, Kim Sung-Dong, Kong Soo-Keun, Lee Seokwhan, Cho Kyu-Sup
Department of Otorhinolaryngology and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
Department of Pathology, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of Korea.
Stem Cells Int. 2020 Feb 3;2020:8595940. doi: 10.1155/2020/8595940. eCollection 2020.
Adipose-derived stromal cells (ADSCs) can repair auricular cartilage defects. Furthermore, stem cell secretome may also be a promising biological therapeutic option, which is equal to or even superior to the stem cell. We explored the therapeutic efficacies of ADSCs and their secretome in terms of rabbit auricular cartilage regeneration. ADSCs and their secretome were placed into surgically created auricular cartilage defects. After 4 and 8 weeks, the resected auricles were histopathologically and immunohistochemically examined. We used real-time PCR to determine the levels of genes expressing collagen type II, transforming growth factor-1 (TGF-1), and insulin-like growth factor-1 (IGF-1). ADSCs significantly improved auricular cartilage regeneration at 4 and 8 weeks, compared to the secretome and PBS groups, as revealed by gross examination, histopathologically and immunohistochemically. ADSCs upregulated the expression of collagen type II, TGF-1, and IGF-1 more so than did the secretome or PBS. The expression levels of collagen type II and IGF-1 were significantly higher at 8 weeks than at 4 weeks after ADSC injection. Although ADSCs thus significantly enhanced new cartilage formation, their secretome did not. Therefore, ADSCs may be more effective than their secretome in the repair of auricular cartilage defect.
脂肪来源的基质细胞（ADSCs）可修复耳廓软骨缺损。此外，干细胞分泌组也可能是一种有前景的生物治疗选择，其等同于甚至优于干细胞。我们探讨了ADSCs及其分泌组在兔耳廓软骨再生方面的治疗效果。将ADSCs及其分泌组植入手术造成的耳廓软骨缺损处。4周和8周后，对切除的耳廓进行组织病理学和免疫组织化学检查。我们使用实时PCR来测定表达II型胶原蛋白、转化生长因子-1（TGF-1）和胰岛素样生长因子-1（IGF-1）的基因水平。大体检查、组织病理学和免疫组织化学结果显示，与分泌组和PBS组相比，ADSCs在4周和8周时显著改善了耳廓软骨再生。ADSCs比分泌组或PBS更能上调II型胶原蛋白、TGF-1和IGF-1的表达。ADSC注射后8周时II型胶原蛋白和IGF-1的表达水平显著高于4周时。因此，尽管ADSCs能显著增强新软骨形成，但其分泌组却不能。所以，在修复耳廓软骨缺损方面，ADSCs可能比其分泌组更有效。
