Dalir S A, Meimandi Parizi A, Tanideh N, Kian M, Nowzari F, Iraji A, Ghaemmagham P, Azarpira N, Zare Sh
Ph.D. Student in Veterinary Surgery, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Vet Res. 2024;25(1):33-40. doi: 10.22099/IJVR.2024.47870.6959.
Mesenchymal stem cell (MSC) therapy has ameliorative effects for treating knee osteoarthritis (KOA) disease. Moreover, there is a growing interest in using MSCs-derived secretome (Sec) containing trophic factors secreted by MSCs for KOA treatment. Recently, some studies have suggested that the combination of MSCs and Sec has the potential to treat the diseases.
This study aimed to evaluate the ameliorative effects of combined administration of infrapatellar fat pad (IPFP)-derived MSCs, a type of adipose-derived stem cells (ASCs), for treating degenerated cartilage in a rat model of KOA.
IPFP-ASCs were isolated from the IPFP of male rats. Sec was obtained from IPFP-ASCs in the fourth passage. Eight weeks after the induction of KOA by collagenase II, the rats were divided into 5 groups (n=5), including a control group with no treatment, and four experimental groups that received sodium hyaluronate (Hyalgan, Hya), ASCs, Sec, and IPFP-ASCs+Sec, respectively by an infrapatellar injection. To perform the pathological and radiological evaluations, the animals were sacrificed 8 weeks later.
Our findings indicated that combined administration of the IPFP-ASCs and Sec statistically (P<0.05) improved scores of medial tibial and femoral condyles and medial fabella osteophytes. Also, it statistically (P<0.05) enhances the cartilage surface, matrix, cell distribution and population viability, and subchondral bone indices. No statistical difference was observed between IPFP-ASCs+Sec and IPFP-ASCs.
Administration of IPFP-ASCs+Sec has a therapeutic potential to treat KOA in rats. However, there is no difference in the combined administration of IPFP-ASCs and Sec with IPFP-ASCs alone.
间充质干细胞（MSC）疗法对治疗膝关节骨关节炎（KOA）具有改善作用。此外，使用含有间充质干细胞分泌的营养因子的间充质干细胞分泌组（Sec）来治疗KOA的兴趣日益增加。最近，一些研究表明，间充质干细胞和分泌组联合使用有可能治疗这些疾病。
本研究旨在评估髌下脂肪垫（IPFP）来源的间充质干细胞（一种脂肪来源的干细胞（ASCs））联合给药对KOA大鼠模型中退变软骨的改善作用。
从雄性大鼠的IPFP中分离出IPFP-ASCs。从第四代IPFP-ASCs中获得分泌组。用胶原酶II诱导KOA八周后，将大鼠分为5组（n = 5），包括未治疗的对照组，以及四个分别通过髌下注射接受透明质酸钠（施沛特，Hya）、ASCs、分泌组和IPFP-ASCs+分泌组的实验组。为了进行病理和放射学评估，8周后处死动物。
我们的研究结果表明，IPFP-ASCs和分泌组联合给药在统计学上（P<0.05）改善了内侧胫骨和股骨髁以及内侧豆状骨骨赘的评分。此外，它在统计学上（P<0.05）增强了软骨表面、基质、细胞分布和群体活力以及软骨下骨指数。IPFP-ASCs+分泌组和IPFP-ASCs之间未观察到统计学差异。
给予IPFP-ASCs+分泌组具有治疗大鼠KOA的潜力。然而，IPFP-ASCs和分泌组联合给药与单独使用IPFP-ASCs没有差异。
