Department of Biomedical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong.
Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, Hong Kong.
Theranostics. 2020 Jan 12;10(5):2215-2228. doi: 10.7150/thno.40146. eCollection 2020.
Adjuvant treatment using local drug delivery is applied in treating glioblastoma multiforme (GBM) after tumor resection. However, there are no non-invasive imaging techniques available for tracking the compositional changes of hydrogel-based drug treatment. : We developed Chemical Exchange Saturation Transfer Magnetic Resonance Imaging (CEST MRI) detectable and injectable liposomal hydrogel to monitor these events at 3T clinical field. Mechanical attributes of these hydrogels and their and CEST imaging properties were systematically studied. : The MRI detectable hydrogels were capable of generating multiparametric readouts for monitoring specific components of the hydrogel matrix simultaneously and independently. Herein, we report, for the first time, CEST contrast at -3.4 ppm provides an estimated number of liposomes and CEST contrast at 5 ppm provides an estimated amount of encapsulated drug. CEST contrast decreased by 1.57% at 5 ppm, while the contrast at -3.4 ppm remained constant over 3 d , demonstrating different release kinetics of these components from the hydrogel matrix Furthermore, histology analysis confirmed that the CEST contrast at -3.4 ppm was associated with liposome concentrations. : This multiparametric CEST imaging of individual compositional changes in liposomal hydrogels, formulated with clinical-grade materials at 3T and described in this study, has the potential to facilitate the refinement of adjuvant treatment for GBM.
辅助治疗使用局部药物输送在肿瘤切除后用于治疗多形性胶质母细胞瘤 (GBM)。然而，目前还没有非侵入性的成像技术可用于跟踪水凝胶药物治疗的组成变化。我们开发了可用于化学交换饱和传递磁共振成像 (CEST MRI) 检测的可注射脂质体水凝胶，以在 3T 临床场中监测这些事件。系统研究了这些水凝胶的机械特性及其 CEST 成像特性。这些 MRI 可检测的水凝胶能够生成多参数读出，以同时且独立地监测水凝胶基质的特定成分。在这里，我们首次报告，CEST 对比在-3.4 ppm 处提供了估计的脂质体数量，CEST 对比在 5 ppm 处提供了包封药物的估计量。在 5 ppm 处，CEST 对比下降了 1.57%，而-3.4 ppm 处的对比在 3 天内保持不变，表明这些成分从水凝胶基质中的释放动力学不同。此外，组织学分析证实，-3.4 ppm 处的 CEST 对比与脂质体浓度相关。本研究中在 3T 下使用临床级材料描述的脂质体水凝胶的这种个体组成变化的多参数 CEST 成像，有可能促进 GBM 辅助治疗的改进。
