Panchal Ketankumar, Koringa Prakash, Sabara Pritesh, Bhatia Dhruv, Jakhesara Subhash, Joshi Chaitanya
1Department of Animal Biotechnology, College of Veterinary Science and Animal Husbundry, Anand Agricultural University, Near Borsad Crossing, Anand, 388001 India.
Gujarat Biotechnology Research Centre, Department of Science and Technology, GoG, Gandhinagar, 382010 India.
3 Biotech. 2020 Mar;10(3):92. doi: 10.1007/s13205-020-2078-9. Epub 2020 Feb 5.
Horn cancer is most prevalent in and poorly defined genetic landscape makes disease diagnosis and treatment difficult. In this study, RNA-Seq and data analysis using CLC Genomics Workbench was employed to identify biomarkers associated with horn cancer. As a result, a total of 149 genes were found significant differentially expressed in horn cancer samples compared to horn normal samples. The study revealed 'keratins' and 'interleukins' as apex groups of significant differentially expressed genes (DEGs). Functional analysis showed that the upregulated keratins support metastasis of tumor via cell proliferation, migration, and affecting cell stability, while downregulated interleukins along with other associated chemokine receptors deprive the immune response to tumor posing clear path for metastasis of horn cancer. Combi-action of both the group facilitates the tumor microenvironment to reproduce tumorigenesis. Analysis of pathways enriched in DEGs and exemplified protein-protein interaction network indicated actual role of DEGs in horn cancer at a fine level. Important effect of deregulated expression of keratin and interleukin genes in horn cancer enrolling their candidacy as potential biomarkers for horn cancer prognosis. This study appraises the possibility to mitigate horn cancer at fine resolution to extract attainable identification of prognostic molecular portraits.
角癌在……中最为普遍，其尚不明确的基因图谱使得疾病的诊断和治疗变得困难。在本研究中，运用RNA测序以及使用CLC基因组学工作台进行数据分析，以鉴定与角癌相关的生物标志物。结果发现，与角正常样本相比，角癌样本中共有149个基因存在显著差异表达。该研究揭示“角蛋白”和“白细胞介素”是显著差异表达基因（DEGs）的顶级类别。功能分析表明，上调的角蛋白通过细胞增殖、迁移以及影响细胞稳定性来支持肿瘤转移，而下调的白细胞介素连同其他相关趋化因子受体则剥夺了对肿瘤的免疫反应，为角癌转移开辟了明确路径。这两类基因的共同作用促进肿瘤微环境重现肿瘤发生过程。对差异表达基因富集的通路分析以及所举例的蛋白质-蛋白质相互作用网络表明，差异表达基因在角癌中发挥着精细层面的实际作用。角蛋白和白细胞介素基因表达失调在角癌中具有重要影响，使其有资格成为角癌预后的潜在生物标志物。本研究评估了在精细分辨率下减轻角癌以获取可实现的预后分子图谱识别的可能性。
