Nitschke K D, Eisenbrandt D L, Lomax L G, Rao K S
Health and Environmental Sciences, Dow Chemical Company, Midland, Michigan 48674.
Fundam Appl Toxicol. 1988 Jul;11(1):60-7. doi: 10.1016/0272-0590(88)90270-9.
Reproductive parameters in Fischer 344 rats were evaluated following inhalation of methylene chloride (MeCl2) for two successive generations. Thirty male and female rats were exposed to 0, 100, 500, or 1500 ppm MeCl2 for 6 hr/day, 5 days/week for 14 weeks and then mated to produce f1 litters. After weaning, 30 randomly selected f1 pups/sex/group were exposed to MeCl2 for 17 weeks and subsequently mated to produce f2 litters. Reproductive parameters examined included fertility, litter size and neonatal growth, and survival. All adults and selected weanlings were examined for grossly visible lesions. Tissues from selected weanlings were examined histopathologically. No adverse effects on reproductive parameters, neonatal survival, or neonatal growth were noted in animals exposed to methylene chloride in either the f0 or f1 generations. Similarly, there were no treatment-related gross pathologic observations in f0 or f1 adults or f1 and f2 weanlings. Histopathologic examination of tissues from f1 and f2 weanlings did not reveal any lesions attributed to methylene chloride. Thus, exposure of rats to concentrations as high as 1500 ppm methylene chloride, which has been shown in a 2-year study to produce treatment-related effects, did not affect any reproductive parameters.
在连续两代吸入二氯甲烷（MeCl2）后，对Fischer 344大鼠的生殖参数进行了评估。30只雄性和雌性大鼠每天暴露于0、100、500或1500 ppm的二氯甲烷中6小时，每周5天，持续14周，然后交配产生F1代幼崽。断奶后，每组随机选择30只F1代幼崽/性别暴露于二氯甲烷中17周，随后交配产生F2代幼崽。检查的生殖参数包括生育力、窝仔数、新生仔鼠生长和存活率。对所有成年大鼠和选定的断奶仔鼠进行肉眼可见病变检查。对选定断奶仔鼠的组织进行组织病理学检查。在F0代或F1代接触二氯甲烷的动物中，未观察到对生殖参数、新生仔鼠存活率或新生仔鼠生长的不良影响。同样，在F0代或F1代成年大鼠以及F1代和F2代断奶仔鼠中，也没有与治疗相关的大体病理观察结果。对F1代和F2代断奶仔鼠组织的组织病理学检查未发现任何归因于二氯甲烷的病变。因此，大鼠暴露于高达1500 ppm的二氯甲烷浓度下（在一项为期两年的研究中已显示会产生与治疗相关的影响），并未影响任何生殖参数。
