Breslin W J, Kirk H D, Streeter C M, Quast J F, Szabo J R
Mammalian and Environmental Toxicology Research Laboratory, Dow Chemical Company, Midland, Michigan 48674.
Fundam Appl Toxicol. 1989 Jan;12(1):129-43. doi: 10.1016/0272-0590(89)90068-7.
This study evaluated the effects of inhaled technical-grade 1,3-dichloropropene (DCPT) on reproduction and neonatal growth and survival. Groups of 30 male and 30 female Fischer 344 rats, approximately 6 weeks of age, were exposed via inhalation to 0, 10, 30 or 90 ppm DCPT for 6 hr/day, 5 days/week, for two generations. The parental f0 and f1 generations were each bred twice. Reproductive and neonatal parameters evaluated included indices of fertility and pup survival, gestation length, litter size, pup body weight, and pup sex ratio. Gross and histologic examinations were concluded on all f0 and f1 adults. In addition, randomly selected f1b and f2b weanlings were given gross examinations. Parental effects were limited to rats exposed to 90 ppm DCPT and included decreased body weights and histopathologic effects on the nasal mucosa of adult male and female rats. The histopathologic effects consisted of slight, focal hyperplasia of the respiratory epithelium and/or focal degenerative changes in the olfactory epithelium. No adverse effects on reproductive parameters or neonatal growth or survival were observed in the f1a, f1b, f2a, or f2b litters even at an exposure concentration which produced effects in adult animals. Based on these results, it is concluded that inhalation exposure of rats up to 90 ppm DCPT for two successive generations did not adversely affect the reproductive and neonatal parameters evaluated.
本研究评估了吸入工业级1,3 - 二氯丙烯（DCPT）对生殖以及新生幼崽生长和存活的影响。将每组30只、约6周龄的雄性和雌性Fischer 344大鼠，通过吸入方式，每天暴露于0、10、30或90 ppm的DCPT中，每周5天，每天6小时，共两代。亲代f0和f1代各繁殖两次。评估的生殖和新生幼崽参数包括生育力指标、幼崽存活率、妊娠期长度、窝仔数、幼崽体重和幼崽性别比例。对所有f0和f1代成年大鼠进行大体和组织学检查。此外，对随机选择的f1b和f2b断奶幼崽进行大体检查。亲代效应仅限于暴露于90 ppm DCPT的大鼠，包括体重减轻以及成年雄性和雌性大鼠鼻粘膜的组织病理学效应。组织病理学效应包括呼吸上皮的轻微局灶性增生和/或嗅上皮的局灶性退行性变化。即使在对成年动物产生影响的暴露浓度下，在f1a、f1b、f2a或f2b窝仔中也未观察到对生殖参数、新生幼崽生长或存活的不良影响。基于这些结果，得出结论：连续两代对大鼠进行高达90 ppm DCPT的吸入暴露，并未对所评估的生殖和新生幼崽参数产生不利影响。
