循环 miR-122 水平与体重减轻和代谢综合征相关。

Levels of Circulating miR-122 are Associated with Weight Loss and Metabolic Syndrome.

机构信息

Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.

Department of Science and Environment, Roskilde University, Roskilde, Denmark.

出版信息

Obesity (Silver Spring). 2020 Mar;28(3):493-501. doi: 10.1002/oby.22704.

DOI:10.1002/oby.22704

PMID:32090516

Abstract

OBJECTIVE

This study investigated whether the levels of specific serum microRNAs (miRNAs) were altered following diet-induced weight loss and whether the serum miRNAs differed in the presence of the metabolic syndrome.

METHODS

The study was a weight loss intervention trial with a prescribed energy deficit of approximately 500 kcal/d. Levels of 22 miRNAs were determined in serum samples from 85 participants with overweight or obesity. miRNAs were analyzed using TaqMan Array miRNA Cards and normalized to the geometric mean of spiked-in ath-miR-159a and U6 small nuclear RNA using the ΔC method.

RESULTS

The average weight loss was 5.7 kg (P < 0.001). miR-122-5p (-0.18 ± 0.06 log fold relative to initial, P < 0.01) and miR-193a-5p (-0.12 ± 0.04, P < 0.01) levels decreased in response to weight loss. miR-126a-3p (0.11 ± 0.04, P = 0.01) and miR-222-3p (1.51 ± 0.12, P < 0.001) levels increased. Furthermore, a higher level of miR-122-5p was observed at baseline in participants with the metabolic syndrome compared with participants without (0.28 ± 0.08, P < 0.01).

CONCLUSIONS

Changes in circulating miR-122-5p, miR-126a-3p, miR-193a-5p, and miR-222-3p in response to diet-induced weight loss are demonstrated. Furthermore, assessment of miR-122-5p could be an indicator of an adverse metabolic health status independent of obesity.

摘要

目的

本研究旨在探讨饮食诱导体重减轻后特定血清 microRNAs（miRNAs）水平是否发生改变，以及代谢综合征存在时血清 miRNAs 是否存在差异。

方法

这是一项减肥干预试验，能量摄入减少约 500 卡路里/天。对 85 名超重或肥胖参与者的血清样本进行了 22 种 miRNAs 的测定。使用 TaqMan Array miRNA 卡进行 miRNA 分析，并使用 ΔC 法，将掺入的 ath-miR-159a 和 U6 小核 RNA 的几何平均值标准化为 miR 的表达量。

结果

平均体重减轻 5.7kg（P<0.001）。miR-122-5p（相对于初始值减少 0.18±0.06log 倍，P<0.01）和 miR-193a-5p（减少 0.12±0.04，P<0.01）水平因体重减轻而降低。miR-126a-3p（增加 0.11±0.04，P=0.01）和 miR-222-3p（增加 1.51±0.12，P<0.001）水平升高。此外，与无代谢综合征的参与者相比，代谢综合征参与者的基线 miR-122-5p 水平更高（0.28±0.08，P<0.01）。