Department of Science and Environment, Roskilde University, 4000 Roskilde, Denmark.
Roskilde Hospital, Region Zealand, 4000 Roskilde, Denmark.
Int J Mol Sci. 2023 Mar 24;24(7):6186. doi: 10.3390/ijms24076186.
Gestational diabetes mellitus (GDM) is a severe pregnancy complication for both the woman and the child. Women who suffer from GDM have a greater risk of developing Type 2 diabetes mellitus (T2DM) later in life. Identification of any potential biomarkers for the early prediction of gestational diabetes can help prevent the disease in women with a high risk. Studies show microRNA (miRNA) as a potential biomarker for the early discovery of GDM, but there is a lack of clarity as to which miRNAs are consistently altered in GDM. This study aimed to perform a systematic review and meta-analysis to investigate miRNAs associated with GDM by comparing GDM cases with normoglycemic controls. The systematic review was performed according to PRISMA guidelines with searches in PubMed, Web of Science, and ScienceDirect. The primary search resulted in a total of 849 articles, which were screened according to the prior established inclusion and exclusion criteria. Following the screening of articles, the review was based on the inclusion of 35 full-text articles, which were evaluated for risk of bias and estimates of quality, after which data were extracted and relative values for miRNAs were calculated. A meta-analysis was performed for the miRNA species investigated in three or more studies: MiR-29a, miR-330, miR-134, miR-132, miR-16, miR-223, miR-155, miR-122, miR-17, miR-103, miR-125, miR-210, and miR-222. While some miRNAs showed considerable between-study variability, miR-29a, miR-330, miR-134, miR-16, miR-223, and miR-17 showed significant overall upregulation in GDM, while circulating levels of miR-132 and miR-155 were decreased among GDM patients, suggesting further studies of these as biomarkers for early GDM discovery.
妊娠期糖尿病（GDM）是一种严重的妊娠并发症，对女性和儿童都有影响。患有 GDM 的女性在以后的生活中患 2 型糖尿病（T2DM）的风险更高。寻找潜在的生物标志物进行早期预测妊娠糖尿病，可以帮助高危女性预防该疾病。研究表明，微小 RNA（miRNA）是早期发现 GDM 的潜在生物标志物，但对于 GDM 中哪些 miRNA 持续改变尚不清楚。本研究旨在通过比较 GDM 病例与血糖正常对照，进行系统评价和荟萃分析，以研究与 GDM 相关的 miRNA。系统评价按照 PRISMA 指南进行，在 PubMed、Web of Science 和 ScienceDirect 中进行检索。初步检索共得到 849 篇文章，根据事先确定的纳入和排除标准进行筛选。在对文章进行筛选后，综述基于纳入的 35 篇全文文章，评估其偏倚风险和质量估计，然后提取数据并计算 miRNA 的相对值。对 miRNA 物种进行了荟萃分析，这些 miRNA 物种在三项或更多研究中进行了调查：miR-29a、miR-330、miR-134、miR-132、miR-16、miR-223、miR-155、miR-122、miR-17、miR-103、miR-125、miR-210 和 miR-222。虽然一些 miRNA 显示出相当大的研究间变异性，但 miR-29a、miR-330、miR-134、miR-16、miR-223 和 miR-17 在 GDM 中表现出明显的总体上调，而 GDM 患者的循环 miR-132 和 miR-155 水平降低，提示进一步研究这些作为早期 GDM 发现的生物标志物。
