Lu Qingyi, Yuan Kai, Li Xiaohong, Jiang Haixu, Huo Guiyang, Jia Wenrui, Huang Guangrui, Xu Anlong
School of Life Sciences, Beijing University of Chinese Medicine.
School of Life Sciences, Beijing University of Chinese Medicine;
J Vis Exp. 2020 Feb 6(156). doi: 10.3791/60543.
Neutrophils are a major member of the innate immune system and play pivotal roles in host defense against pathogens and pathologic inflammatory reactions. Neutrophils can be recruited to inflammation sites via the guidance of cytokines and chemokines. Overwhelming infiltration of neutrophils can lead to indiscriminate tissue damage, such as in rheumatoid arthritis (RA). Neutrophils isolated from peritoneal exudate respond to a defined chemoattractant, N-formyl-Met-Leu-Phe (fMLP), in vitro in Transwell or Zigmond chamber assays. The air pouch experiment can be used to evaluate the chemotaxis of neutrophils towards lipopolysaccharide (LPS) in vivo. The adjuvant-induced arthritis (AA) mouse model is frequently used in RA research, and immunohistochemical staining of joint sections with anti-myeloperoxidase (MPO) or anti-neutrophil elastase (NE) antibodies is a well-established method to measure neutrophil infiltration. These methods can be used to discover promising therapies targeting neutrophil migration.
中性粒细胞是先天性免疫系统的主要成员，在宿主抵御病原体和病理性炎症反应中起关键作用。中性粒细胞可通过细胞因子和趋化因子的引导被招募到炎症部位。中性粒细胞的大量浸润会导致不加区分的组织损伤，如在类风湿性关节炎（RA）中。从腹膜渗出液中分离出的中性粒细胞在体外Transwell或Zigmond小室试验中对特定的趋化因子N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）有反应。气袋实验可用于评估体内中性粒细胞对脂多糖（LPS）的趋化性。佐剂诱导的关节炎（AA）小鼠模型常用于RA研究，用抗髓过氧化物酶（MPO）或抗中性粒细胞弹性蛋白酶（NE）抗体对关节切片进行免疫组织化学染色是一种成熟的测量中性粒细胞浸润的方法。这些方法可用于发现针对中性粒细胞迁移的有前景的治疗方法。
