Jiang Ning, Fan Xinlei, Tian Chengming, Crous Pedro W
The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China.
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Mycologia. 2020 Mar-Apr;112(2):267-292. doi: 10.1080/00275514.2019.1698925. Epub 2020 Feb 24.
The Diaporthales (Sordariomycetes) includes many important families of plant pathogenic fungi, such as the notorious Cryphonectriaceae. The aim of the present study was to reevaluate this family, along with other families in Diaporthales. Based on phylogenetic analyses using combined sequence data of the internal transcribed spacer (ITS) region, large subunit of the nrDNA (28S), and the translation elongation factor 1-alpha () and DNA-directed RNA polymerase II second largest subunit () genes, Cryphonectriaceae is separated into two subclades, comprising 21 genera and 55 species. Foliocryphiaceae, fam. nov., is morphologically and phylogenetically its closest relative but is distinct due to its phylogeny and dimorphic conidia. Mastigosporellaceae, fam. nov., is distinguished from other families in Diaporthales by owning apical conidial appendages. , gen. nov., within the family Foliocryphiaceae, with two species associated with canker in China, is characterized by fusoid, aseptate macroconidia. Additionally, two new combinations are proposed, namely, , based on , and , based on . Based on results obtained in this study, 31 families are accepted into Diaporthales.
座囊菌目（粪壳菌纲）包含许多重要的植物病原真菌家族，比如臭名昭著的隐孢壳科。本研究的目的是重新评估该科以及座囊菌目中的其他科。基于对内部转录间隔区（ITS）区域、nrDNA的大亚基（28S）、翻译延伸因子1-α（）和DNA指导的RNA聚合酶II第二大亚基（）基因的联合序列数据进行的系统发育分析，隐孢壳科被分为两个亚分支，包含21个属和55个种。新科叶隐孢壳科在形态学和系统发育上是其最亲近的亲属，但因其系统发育和二型分生孢子而不同。新科鞭孢壳科在座囊菌目中与其他科的区别在于其分生孢子顶端有附属物。叶隐孢壳科内的新属，在中国有两个与溃疡病相关的物种，其特征是梭形、无隔的大分生孢子。此外，还提出了两个新组合，即基于的，以及基于的。根据本研究获得的结果，座囊菌目认可31个科。
