Téteau Ophélie, Jaubert Manon, Desmarchais Alice, Papillier Pascal, Binet Aurélien, Maillard Virginie, Elis Sébastien
PRC, INRAE, CNRS, Université de Tours, IFCE, Nouzilly, France.
Service de Chirurgie pédiatrique viscérale, urologique, plastique et brûlés, Tours, France.
Reproduction. 2020 May;159(5):571-583. doi: 10.1530/REP-19-0575.
Bisphenols, plasticisers used in food containers, can transfer to food. Bisphenol A (BPA) has been described as an endocrine disruptor and consequently banned from the food industry in several countries. It was replaced by a structural analogue, Bisphenol S (BPS). BPA action on the steroidogenesis is one of the mechanisms underlying its adverse effects on the efficiency of female reproduction. This study aimed to determine whether BPS is a safe alternative to BPA regarding GC functions. Antral follicles (2-6 mm), of approximatively 1000 adult ewe ovaries, were aspired and GC purified. For 48 h, ovine GC were treated with BPA or BPS (from 1 nM to 200 µM) and the effects on cell viability, proliferation, steroid production, steroidogenic enzyme expression and signalling pathways were investigated. Dosages at and greater than 100 μM BPA and 10 µM BPS decreased progesterone secretion by 39% (P < 0.001) and 22% (P = 0.040), respectively. BPA and BPS 10 μM and previously mentioned concentrations increased oestradiol secretion two-fold (P < 0.001 and P = 0.082, respectively). Only 100 µM BPA induced a decrease (P < 0.001) in gene expression of the enzymes of steroidogenesis involved in the production of progesterone. BPA reduced MAPK3/1 phosphorylation and ESR1 and ESR2 gene expression, effects that were not observed with BPS. BPA and BPS altered steroidogenesis of ovine GC. Thus, BPS does not appear to be a safe alternative for BPA. Further investigations are required to elucidate BPA and BPS mechanisms of action.
双酚类物质是食品容器中使用的增塑剂，可迁移至食品中。双酚A（BPA）被描述为一种内分泌干扰物，因此在多个国家被食品行业禁用。它被其结构类似物双酚S（BPS）所取代。BPA对类固醇生成的作用是其对雌性生殖效率产生不利影响的潜在机制之一。本研究旨在确定就颗粒细胞（GC）功能而言，BPS是否是BPA的安全替代品。从约1000只成年母羊卵巢中抽吸直径为2 - 6毫米的窦卵泡，并纯化GC。将羊GC用BPA或BPS（浓度从1 nM至200 μM）处理48小时，研究其对细胞活力、增殖、类固醇生成、类固醇生成酶表达及信号通路的影响。100 μM及以上剂量的BPA和10 μM的BPS分别使孕酮分泌减少39%（P < 0.001）和22%（P = 0.040）。10 μM的BPA和BPS以及上述浓度使雌二醇分泌增加两倍（分别为P < 0.001和P = 0.082）。仅100 μM的BPA导致参与孕酮生成的类固醇生成酶基因表达下降（P < 0.001）。BPA降低了MAPK3/1磷酸化以及ESR1和ESR2基因表达，而BPS未观察到这些效应。BPA和BPS改变了羊GC的类固醇生成。因此，BPS似乎不是BPA的安全替代品。需要进一步研究以阐明BPA和BPS的作用机制。
