UMR PRC, CNRS, IFCE, INRAE, Université de Tours, 37380 Nouzilly, France.
CHRU de Tours, 37000 Tours, France.
Int J Mol Sci. 2020 Feb 12;21(4):1238. doi: 10.3390/ijms21041238.
Bisphenol A (BPA) is a widespread compound in the plastic industry that is especially used to produce baby bottles, food packaging and metal cans. BPA, an endocrine disruptor, leads to alterations in reproductive function and therefore has been banned from the food industry. Unregulated BPA analogues, particularly Bisphenol S (BPS), have emerged and are now used in the plastic industry. Thus, this study aimed to examine the acute effects of low and environmental doses of BPS on ewe oocyte quality and developmental competence, and its mechanism of action, during in vitro maturation.
Ewe cumulus-oocyte complexes underwent in vitro maturation in the presence or absence of BPS (1 nM, 10 nM, 100 nM, 1 µM or 10 µM). Oocytes were then subjected to in vitro fertilisation and development.
1 µM BPS induced a 12.7% decrease in the cleavage rate ( = 0.004) and a 42.6% decrease in the blastocyst rate ( = 0.017) compared to control. The blastocyst rate reduction was also observed with 10 nM BPS. Furthermore, 10 µM BPS reduced the oocyte maturation rate, and 1 µM BPS decreased cumulus cell progesterone secretion. PR and AMH gene expression were reduced in cumulus cells. BPS induced a 5-fold increase in MAPK 3/1 activation ( = 0.04).
BPS impaired ewe oocyte developmental competence. The data suggest that BPS might not be a safe BPA analogue. Further studies are required to elucidate its detailed mechanism of action.
双酚 A（BPA）是塑料工业中广泛存在的化合物，尤其用于生产婴儿奶瓶、食品包装和金属罐。双酚 A 是一种内分泌干扰物，会导致生殖功能改变，因此已被禁止用于食品工业。未受监管的双酚 A 类似物，特别是双酚 S（BPS），已经出现并在塑料工业中使用。因此，本研究旨在研究低剂量和环境剂量的 BPS 对绵羊卵母细胞质量和发育能力的急性影响，以及其在体外成熟过程中的作用机制。
绵羊卵丘-卵母细胞复合物在存在或不存在 BPS（1 nM、10 nM、100 nM、1 µM 或 10 µM）的情况下进行体外成熟。然后对卵母细胞进行体外受精和发育。
与对照组相比，1 µM BPS 诱导卵裂率降低 12.7%（ = 0.004），囊胚率降低 42.6%（ = 0.017）。10 nM BPS 也观察到囊胚率降低。此外，10 µM BPS 降低卵母细胞成熟率，1 µM BPS 降低卵丘细胞孕激素分泌。PR 和 AMH 基因在卵丘细胞中的表达减少。BPS 诱导 MAPK 3/1 激活增加 5 倍（ = 0.04）。
BPS 损害绵羊卵母细胞的发育能力。数据表明，BPS 可能不是安全的 BPA 类似物。需要进一步研究阐明其详细的作用机制。
