CAS Key Laboratory for Biomedical Effects of Nanomaterial and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China.
Molecules. 2020 Feb 19;25(4):919. doi: 10.3390/molecules25040919.
The intestinal epithelium is a major barrier that limits the absorption of oral drugs. The integrity of the epithelial tissue is a very important factor for preventing intestinal diseases. However, destabilization of the epithelium can promote the transportation of nanocarriers and increase the absorption of oral drugs. In our research, three different gold nanoparticles (GNPs) of the same size but with differing negative surface charge were designed and constructed as a model to determine the surface properties crucial for promoting absorptivity and bioavailability of the nanocarriers. The higher the ratio of surface carboxyl groups on GNPs, the higher capacity to induce transepithelial electrical resistance change and cell monolayer tight junction opening with higher permeability. The half carboxyl and half methyl surfaced GNPs displayed unique zonal surface patterns exhibited the greater ability to pass through intestinal epithelial cell layer but had a relatively small influence on tight junction distribution.
肠上皮是限制口服药物吸收的主要屏障。上皮组织的完整性对于预防肠道疾病非常重要。然而，上皮细胞的不稳定会促进纳米载体的运输，并增加口服药物的吸收。在我们的研究中，设计并构建了三种具有相同尺寸但带不同负表面电荷的不同金纳米颗粒（GNPs）作为模型，以确定对促进纳米载体的吸收性和生物利用度至关重要的表面特性。GNPs 表面羧基比例越高，诱导跨上皮电阻变化和细胞单层紧密连接开放的能力越高，通透性也越高。具有半羧基和半甲基表面的 GNPs 表现出独特的分区表面模式，具有更强的穿过肠上皮细胞层的能力，但对紧密连接分布的影响相对较小。
