Wlazło Anna, Święcicka Magdalena, Koter Marek D, Krępski Tomasz, Bolibok Leszek, Stochmal Anna, Kowalczyk Mariusz, Rakoczy-Trojanowska Monika
Department of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Department of Silviculture, Institute of Forest Sciences, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Genes (Basel). 2020 Feb 20;11(2):223. doi: 10.3390/genes11020223.
Two genes, and , both encoding indole-3-glycerol phosphate lyase (IGL), are believed to control the conversion of indole-3-glycerol phosphate (IGP) to indole. The first of these has generally been supposed to be regulated developmentally, being expressed at early stages of plant development with the indole being used in the benzoxazinoid (BX) biosynthesis pathway. In contrast, it has been proposed that the second one is regulated by stresses and that the associated free indole is secreted as a volatile. However, our previous results contradicted this. In the present study, we show that the gene takes over the role of at later developmental stages, between the 42nd and 70th days after germination. In the majority of plants with silenced expression, was either expressed at a significantly higher level than or it was the only gene with detectable expression. Therefore, we postulate that the synthesis of indole used in BX biosynthesis in rye is controlled by both and , which are both regulated developmentally and by stresses. In silico and in vivo analyses of the promoter sequences further confirmed our hypothesis that the roles and modes of regulation of the and genes are similar.
两个基因， 和 ，均编码吲哚 - 3 - 甘油磷酸裂解酶（IGL），被认为控制着吲哚 - 3 - 甘油磷酸（IGP）向吲哚的转化。其中第一个基因通常被认为受发育调控，在植物发育早期表达，吲哚用于苯并恶嗪类（BX）生物合成途径。相比之下，有人提出第二个基因受胁迫调控，相关的游离吲哚以挥发性物质形式分泌。然而，我们之前的结果与此相矛盾。在本研究中，我们表明 基因在萌发后第42天至70天的后期发育阶段接管了 基因的作用。在大多数 表达沉默的植物中， 要么表达水平显著高于 ，要么是唯一有可检测表达的基因。因此，我们推测黑麦中用于BX生物合成的吲哚合成受 和 共同控制，这两个基因均受发育和胁迫调控。对启动子序列的电子分析和体内分析进一步证实了我们的假设，即 和 基因的作用及调控模式相似。
