Rakoczy-Trojanowska Monika, Orczyk Wacław, Krajewski Paweł, Bocianowski Jan, Stochmal Anna, Kowalczyk Mariusz
Warsaw University of Life Sciences, Warsaw, Poland.
Centre For Biological Diversity Conservation in Powsin, Polish Academy of Sciences Botanical Garden, Warsaw, Poland.
J Appl Genet. 2017 Feb;58(1):1-9. doi: 10.1007/s13353-016-0356-3. Epub 2016 Jul 27.
Hydroxamates (HX) are major secondary metabolites synthesized by rye and are responsible for some of the unique properties of this cereal, including good tolerance of biotic and abiotic stresses and allelopathy. Recently, five genes encoding enzymes taking part in HX biosynthesis have been sequenced and characterized, which was the starting point to undertake the present study. Association analysis of the content of six HX-HBOA (2-hydroxy-1,4-benzoxazin-3-one), GDIBOA (2,4-dihydroxy-1,4-benzoxazin-3(4H)-one glucoside), DIBOA (2,4-dihydroxy-1,4-benzoxazin-3(4H)-one), GDIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3(4H)-one glucoside), DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3(4H)-one) and MBOA (6-methoxy-benzoxazolin-2(3H)-one) in the above-ground parts of plants and roots was performed on a population consisting of 102 and 121 diverse inbred lines, in 2013 and 2014, respectively. Altogether, 48 single nucleotide polymorphisms (SNPs) were found to be associated with the content of at least one HX: 20 SNPs were associated with HX synthesized in the above-ground parts of rye plants (AG-SNP), and 28 were associated with HX synthesized in the roots (R-SNP). The highest number of SNPs was present in genes ScBx1 (9) and ScBx5 (11). The majority of SNPs were affected by environmental factors, except for two: ScBx4_1702 associated with GDIBOA and MBOA contents, and ScBx5_1105 associated with HBOA content in roots.
异羟肟酸（HX）是黑麦合成的主要次生代谢产物，赋予了这种谷物一些独特的特性，包括对生物和非生物胁迫的良好耐受性以及化感作用。最近，参与HX生物合成的五个编码酶的基因已被测序和表征，这是开展本研究的起点。分别于2013年和2014年，对由102个和121个不同自交系组成的群体进行了植物地上部分和根部中六种HX的含量关联分析，这六种HX分别是2-羟基-1,4-苯并恶唑-3-酮（HX-HBOA）、2,4-二羟基-1,4-苯并恶唑-3(4H)-酮葡萄糖苷（GDIBOA）、2,4-二羟基-1,4-苯并恶唑-3(4H)-酮（DIBOA）、2,4-二羟基-7-甲氧基-1,4-苯并恶唑-3(4H)-酮葡萄糖苷（GDIMBOA）、2,4-二羟基-7-甲氧基-1,4-苯并恶唑-3(4H)-酮（DIMBOA）和6-甲氧基-苯并恶唑啉-2(3H)-酮（MBOA）。总共发现48个单核苷酸多态性（SNP）与至少一种HX的含量相关：20个SNP与黑麦植物地上部分合成的HX相关（AG-SNP），28个与根部合成的HX相关（R-SNP）。SNP数量最多的基因是ScBx1（9个）和ScBx5（11个）。除了两个SNP外，大多数SNP都受环境因素影响，这两个SNP分别是与GDIBOA和MBOA含量相关的ScBx4_1702，以及与根部HBOA含量相关的ScBx5_1105。
