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BAK1 通过光强度介导磷酸化并激活过氧化氢酶来调节植物生长和发育。

BAK1 Mediates Light Intensity to Phosphorylate and Activate Catalases to Regulate Plant Growth and Development.

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.

Department of Plant Sciences, University of Idaho, Moscow, ID 83844, USA.

出版信息

Int J Mol Sci. 2020 Feb 20;21(4):1437. doi: 10.3390/ijms21041437.

DOI:10.3390/ijms21041437
PMID:32093294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7073115/
Abstract

BAK1 (brassinosteroid-insensitive 1 (BRI1) associated receptor kinase 1) plays major roles in multiple signaling pathways as a coreceptor to regulate plant growth and development and stress response. However, the role of BAK1 in high light signaling is still poorly understood. Here we observed that overexpression of BAK1 in Arabidopsis interferes with the function of high light in promoting plant growth and development, which is independent of the brassinosteroid (BR) signaling pathway. Further investigation shows that high light enhances the phosphorylation of BAK1 and catalase activity, thereby reducing hydrogen peroxide (HO) accumulation. Catalase3 (CAT3) is identified as a BAK1-interacting protein by affinity purification and LC-MS/MS analysis. Biochemical analysis confirms that BAK1 interacts with and phosphorylates all three catalases (CAT1, CAT2, and CAT3) of Arabidopsis genome, and the trans-phosphorylation sites of three catalases with BAK1-CD are identified by LC-MS/MS in vitro. Genetic analyses reveal that the BAK1 overexpression plants knocked out all the three genes completely abolishing the effect of BAK1 on suppression of high light-promoted growth. This study first unravels the role of BAK1 in mediating high light-triggered activation of CATs, thereby degrading HO and regulating plant growth and development in Arabidopsis.

摘要

BAK1（油菜素内酯不敏感 1（BRI1）相关受体激酶 1）作为核心受体，在多个信号通路中发挥重要作用，调节植物的生长发育和应激反应。然而，BAK1 在高光信号中的作用仍知之甚少。在这里，我们观察到拟南芥中 BAK1 的过表达干扰了高光促进植物生长和发育的功能，这与油菜素内酯（BR）信号通路无关。进一步的研究表明，高光增强了 BAK1 的磷酸化和过氧化氢酶活性，从而减少了过氧化氢（HO）的积累。过氧化氢酶 3（CAT3）通过亲和纯化和 LC-MS/MS 分析被鉴定为 BAK1 的互作蛋白。生化分析证实 BAK1 与拟南芥基因组中的三种过氧化氢酶（CAT1、CAT2 和 CAT3）相互作用并磷酸化它们，并且通过体外 LC-MS/MS 鉴定了 BAK1-CD 与三种过氧化氢酶的转磷酸化位点。遗传分析表明，BAK1 过表达植物敲除了所有三个基因，完全消除了 BAK1 对抑制高光促进生长的影响。这项研究首次揭示了 BAK1 在介导高光触发的 CATs 激活中的作用，从而降解 HO 并调节拟南芥的生长发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/fcef7c54b046/ijms-21-01437-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/dd40661f02e2/ijms-21-01437-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/ce60c1d110b9/ijms-21-01437-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/2f935bf68b53/ijms-21-01437-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/890fa1c626b9/ijms-21-01437-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/f1934a5c8b5a/ijms-21-01437-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/800c78532891/ijms-21-01437-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/fcef7c54b046/ijms-21-01437-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/dd40661f02e2/ijms-21-01437-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/ce60c1d110b9/ijms-21-01437-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/2f935bf68b53/ijms-21-01437-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/890fa1c626b9/ijms-21-01437-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/f1934a5c8b5a/ijms-21-01437-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/800c78532891/ijms-21-01437-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f180/7073115/fcef7c54b046/ijms-21-01437-g007.jpg

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