Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
Int J Mol Sci. 2020 Feb 20;21(4):1453. doi: 10.3390/ijms21041453.
Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer's recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production.
白藜芦醇是一种植物多酚，是一种常用的马用营养补充剂，据称具有抗炎作用。目前尚未有研究报道口服白藜芦醇对马的肿瘤坏死因子（TNF）、白细胞介素-1β（IL-1β）、白细胞吞噬活性或氧化爆发功能的影响。本研究的目的是确定一种市售的口服白藜芦醇产品对健康成年马固有免疫功能的影响。在接受白藜芦醇或安慰剂治疗 3 周前后，从 12 匹马中采集全血。在治疗组之间，比较治疗前后吞噬作用、氧化爆发和病原体相关分子模式（PAMP）基序刺激白细胞产生 TNF 和 IL-1β的情况。通过流式细胞术评估吞噬作用和氧化爆发能力。使用细胞毒性和 ELISA 测定法分别测定 TNF 和 IL-1β。白藜芦醇和安慰剂治疗组之间的吞噬作用、氧化爆发或刺激 TNF 或 IL-1β产生均无显著差异。在 3 周的常规推荐剂量下口服白藜芦醇不会显著影响吞噬活性、氧化爆发功能或 PAMP 刺激的白细胞细胞因子产生。
