School of Pharmacy, Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine, Hefei, China.
The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.
J Pharmacol Sci. 2020 May;143(1):1-8. doi: 10.1016/j.jphs.2020.02.004. Epub 2020 Feb 13.
To investigated the metrorrhagia volume-reduction activity, anti-inflammatory activity and repair-promoting activity of Clinopodium chinense (Benth.) O. Kuntze.
An abnormal uterine bleeding (AUB) model was induced via oral administration of mifepristone and misoprostol to pregnant rats, which were treated with the total extract of C. chinense (TEC). After 7 days, the metrorrhagia volume was measured, the levels of TXB2, 6-keto-PGF1α, IL-6 and TNF-α were measured by ELISA, the pathological changes and micro vessel density (MVD) of the endometrium were evaluated using HE and immunofluorescence staining, and the expression of VEGF, MMP-2/9 and TGF-β were assessed by Western blotting. Preliminary phytochemicals were screened and identified by UPLC-Q-TOF-MS.
Eleven compounds in C. chinense were identified via comparison to standard substances. The results of animal experiment showed TEC could reduce metrorrhagia volume, alleviate pathological injury and increase MVD to promote recovery of the endometrium; TEC could also increase the levels of TXB2 and the expression of VEGF, TGF-β, decrease the levels of IL-6, TNF-α and the expression of MMP-2/9.
TEC showed beneficial effects on treating AUB by reducing metrorrhagia volume, inhibiting the inflammatory response and promoting the repair of the endometrium. Additionally, TEC also showed great haemostatic potential in AUB.
研究筋骨草（Benth.）O. Kuntze 的减少子宫出血体积、抗炎和促进修复活性。
通过给怀孕大鼠口服米非司酮和米索前列醇诱导异常子宫出血（AUB）模型，用筋骨草总提取物（TEC）处理。7 天后，测量子宫出血体积，通过 ELISA 测量 TXB2、6-酮-PGF1α、IL-6 和 TNF-α 的水平，通过 HE 和免疫荧光染色评估子宫内膜的病理变化和微血管密度（MVD），通过 Western blot 评估 VEGF、MMP-2/9 和 TGF-β的表达。通过 UPLC-Q-TOF-MS 筛选和鉴定初步的植物化学物质。
通过与标准物质比较，从筋骨草中鉴定出 11 种化合物。动物实验结果表明，TEC 可减少子宫出血体积，减轻病理损伤，增加 MVD 以促进子宫内膜恢复；TEC 还可以增加 TXB2 和 VEGF、TGF-β的水平，降低 IL-6、TNF-α 和 MMP-2/9 的表达。
TEC 通过减少子宫出血体积、抑制炎症反应和促进子宫内膜修复，对治疗 AUB 具有有益作用。此外，TEC 在 AUB 中也表现出很强的止血潜力。
