Zhong Suqin, Qian Lichao, Tan Yong
Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210029, China.
The Chinese Traditional Medicine Department of Ganzhou Maternity & Child Health Hospital, Ganzhou, Jiangxi, 341000, China.
Heliyon. 2024 Apr 20;10(9):e30079. doi: 10.1016/j.heliyon.2024.e30079. eCollection 2024 May 15.
To investigate the protective effects against abnormal uterine bleeding (AUB) and possible mechanisms of Xue Ping tablets (XPT) using a rat model.
A total of 58 unmated female and 25 male SPF SD rats aged 8-9 weeks were selected. Eight unmated female rats were selected as the blank control group according to the complete random method. The other 50 rats were mated in a female/male ratio of 2:1. In the morning after mating, vaginal smears were collected. Presence of vaginal plug or sperm was regarded as the first day of pregnancy. All pregnant rats were given 8.3 mg/kg of mifepristone by gavage at 8:00 a.m. and 100 μg/kg misoprostol by gavage at 6:00 p.m. on the seventh day of pregnancy to induce incomplete abortion, thereby establishing a rat model of AUB. Forty rats were randomly divided into model, low- (220 mg/kg), medium- (441 mg/kg), high-dose (882 mg/kg) XPT, and positive control groups. The positive group was given 130 mg/kg Gong Xue Ning (GXN). The model group and the blank group were given an equal amount of distilled water.
Compared with the model group, the volume of bleeding in the positive and middle- and high-dose XPT groups decreased ( < 0.05). Moreover, compared with the model group, the progesterone levels in the positive and XPT groups were significantly increased. Immunohistochemistry showed that XPT significantly decreased the expression levels of VEGF, -ERK, NF-κB, SAA, MMP-2, MMP-9, TIMP-1, TIMP-2 and TIMP-3. WB results showed that XPT significantly decreased the expression levels of -ERK, MMP-9, NF-κB, MMP-2 and VEGF. QRT-PCR results showed that XPT significantly decreased the expression levels of VEGF, NF-κB, SAA, MMP-2, TIMP-1, TIMP-2 and TIMP-3 ( < 0.05).
XPT could reduce AUB by inhibiting the inflammatory factors involved in the VEGF-ERK1/2 pathway.
采用大鼠模型研究血平片（XPT）对异常子宫出血（AUB）的保护作用及其可能机制。
选取58只8 - 9周龄未交配的雌性SPF级SD大鼠和25只雄性SPF级SD大鼠。按完全随机法选取8只未交配的雌性大鼠作为空白对照组。将其余50只大鼠按2:1的雌雄性比例进行交配。交配后次日晨，采集阴道涂片。出现阴道栓或精子视为妊娠第1天。所有妊娠大鼠于妊娠第7天上午8:00经口灌胃给予8.3 mg/kg米非司酮，下午6:00经口灌胃给予100 μg/kg米索前列醇以诱导不完全流产，从而建立AUB大鼠模型。将40只大鼠随机分为模型组、低剂量（220 mg/kg）、中剂量（441 mg/kg）、高剂量（882 mg/kg）XPT组和阳性对照组。阳性组给予130 mg/kg宫血宁（GXN）。模型组和空白组给予等量蒸馏水。
与模型组相比，阳性组及XPT中、高剂量组出血量减少（P < 0.05）。此外，与模型组相比，阳性组和XPT组孕酮水平显著升高。免疫组化显示，XPT显著降低VEGF、p-ERK、NF-κB、SAA、MMP-2、MMP-9、TIMP-1、TIMP-2和TIMP-3的表达水平。WB结果显示，XPT显著降低p-ERK、MMP-9、NF-κB、MMP-2和VEGF的表达水平。QRT-PCR结果显示，XPT显著降低VEGF、NF-κB、SAA、MMP-2、TIMP-1、TIMP-2和TIMP-3的表达水平（P < 0.05）。
XPT可通过抑制VEGF-ERK1/2途径中的炎症因子来减少AUB。
