Department of Biology, Queen's University, Kingston, ON, K7L-3N6, Canada.
Department of Chemistry, Queen's University, Kingston, ON, K7L-3N6, Canada.
Sci Rep. 2020 Feb 24;10(1):3267. doi: 10.1038/s41598-020-60245-6.
The isomerisation of azo dyes can induce conformational changes which have potential applications in medicine and environmental protection. We developed an agar diffusion assay to test the capture and release of biologically active molecules from an azo electro-optic polymer, Poly (Disperse Red 1 methacrylate) (DR1/PMMA). The assay monitors the growth of bacteria placed in soft agar under a glass coverslip. Antibiotics can then be applied on the coverslip resulting in the clearance of the area under the coverslip due to growth inhibition. This assay demonstrates that DR1/PMMA is able to capture either tetracycline or ampicillin and the relative amount of DR1/PMMA required for capture was determined. Finally, the active antibiotics can be released from DR1/PMMA by exposure to green laser light. Exposure to white light from a torch or to heat does not release the antibiotic.
偶氮染料的异构化可以诱导构象变化，这在医学和环境保护中有潜在的应用。我们开发了一种琼脂扩散测定法来测试从偶氮电光聚合物聚（分散红 1 甲基丙烯酸酯）（DR1/PMMA）中捕获和释放具有生物活性的分子。该测定法监测置于玻璃盖玻片下软琼脂中的细菌的生长。然后可以在盖玻片上施加抗生素，导致由于生长抑制而清除盖玻片下的区域。该测定法表明，DR1/PMMA 能够捕获四环素或氨苄青霉素，并且确定了捕获所需的 DR1/PMMA 的相对量。最后，可以通过暴露于绿光将活性抗生素从 DR1/PMMA 中释放出来。用火炬发出的白光或加热不能释放抗生素。
