Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Biosensors (Basel). 2022 Jan 28;12(2):78. doi: 10.3390/bios12020078.
In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for CPPU was optimized, which exhibits an IC of 1.04 ng/mL, a limit of detection of 0.16 ng/mL, and a linear range of 0.31-3.43 ng/mL for CPPU. Cross-reactivity percentages with six analogues were all below 6%. The average recovery rates for cucumber and orange samples were from 85.23% to 119.14%. The analysis results of this icELISA showed good consistency with those from liquid chromatography mass spectrometry. These results suggest that the proposed icELISA provides a sensitive, specific, and reliable strategy for CPPU detection in food samples.
在这项研究中，氯吡脲（CPPU）与琥珀酸酐偶联生成 CPPU 半抗原（CPPU-COOH），并制备出可特异性识别 CPPU 的高亲和力单克隆抗体（mAb）。使用该 mAb 作为识别试剂，优化了一种灵敏的间接竞争酶联免疫吸附测定法（icELISA）用于 CPPU 的检测，其对 CPPU 的 IC为 1.04ng/mL，检出限为 0.16ng/mL，线性范围为 0.31-3.43ng/mL。与六种类似物的交叉反应百分比均低于 6%。黄瓜和橙子样品的平均回收率在 85.23%到 119.14%之间。icELISA 的分析结果与液相色谱质谱的分析结果具有良好的一致性。这些结果表明，所提出的 icELISA 为食品样品中 CPPU 的检测提供了一种灵敏、特异、可靠的策略。
