Abdullah Hend H A M, Hussein Hany A, El-Razik Khaled A Abd, Barakat Ashraf M A, Soliman Yousef A
Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.
Department of Animal Reproduction and Artificial Insemination, National Research Centre, Dokki, Giza, Egypt.
Vet World. 2019 Dec;12(12):1945-1950. doi: 10.14202/vetworld.2019.1945-1950. Epub 2019 Dec 12.
Q fever is a zoonotic disease caused by . Cattle, sheep, and goat are the main reservoir of . In Egypt, the epidemiological data about in camels are limited. Therefore, the current study was conducted to identify infection in camels by different molecular tools and to estimate its seropositivity through the detection of anti-C. burnetii antibodies in camel sera.
Blood samples were collected 112 from camels in Giza and Cairo Provinces, Egypt. All blood samples were screened by trans-quantitative polymerase chain reaction (trans-qPCR) for and positive samples subjected to standard PCR using the superoxide dismutase enzyme coding gene of . Sera of studied camels were examined for the presence of antibodies against using enzyme-linked immunosorbent assay.
Out of 112 camels, 19 were positive for by qPCR with an overall prevalence of 16.9% (18.6% in Giza and 15.1% in Cairo Provinces, respectively). The seroprevalence of anti- IgG antibodies in the examined camels was 4.5% (5/112).
Trans-qPCR assay is a rapid and sensitive tool for the detection of in acute stage. Camels should be considered one of the major reservoirs for in Egypt.
Q热是一种由……引起的人畜共患病。牛、羊和山羊是……的主要宿主。在埃及，关于骆驼感染……的流行病学数据有限。因此，本研究旨在通过不同的分子工具鉴定骆驼中的……感染，并通过检测骆驼血清中的抗伯氏考克斯体抗体来估计其血清阳性率。
从埃及吉萨省和开罗省的112头骆驼采集血样。所有血样通过转录定量聚合酶链反应（trans-qPCR）筛查……，阳性样本使用……的超氧化物歧化酶编码基因进行标准PCR。使用酶联免疫吸附试验检测所研究骆驼血清中抗……抗体的存在。
在112头骆驼中，19头通过qPCR检测为……阳性，总体患病率为16.9%（吉萨省为18.6%，开罗省为15.1%）。在所检测骆驼中抗……IgG抗体的血清阳性率为4.5%（5/112）。
Trans-qPCR检测是急性期检测……的快速且灵敏的工具。骆驼应被视为埃及……的主要宿主之一。
