Jafarian Amir Hossein, Mohamadian Roshan Nema, Ayatollahi Hossein, Omidi Abbas Ali, Ghaznavi Masoumeh, Gharib Masoumeh
Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Hematology and Blood Bank, Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Pathol. 2020 Winter;15(1):30-33. doi: 10.30699/IJP.2019.77233.1728.
BACKGROUND & OBJECTIVE: Idiopathic pulmonary fibrosis (IPF) is a chronic and uniformly fatal interstitial lung disease with incompletely understood pathogenesis. Several studies have given the evidence for and against viral cofactors in the pathogenesis of Idiopathic pulmonary fibrosis. In this study (EBV) and (HHV-8) have been studied for a possible role in the pathogenesis of IPF.
Polymerase chain reaction (PCR) was employed for the detection of EBV and HHV-8 in 58 formalin-fixed paraffin-embedded lung tissue specimens (29 controls and 29 IPF specimens).
EBV DNA was present in the lung tissue of 6 out of 29 (20.7%) IPF specimens compared with 1 out of 29 (3.4%) controls (=0.102). The HHV-8 gene was identified in 3 out of 29 (10.3%) cases of IPF specimens. The control group showed no evidence of HHV-8 gene (=0.227).
Although multiple studies are strongly suggestive of a role for EBV and HHV-8 in the development of IPF, there was no statistically significant difference in the prevalence of EBV and HHV-8 DNA in the IPF specimens and controls in this study.
特发性肺纤维化（IPF）是一种慢性且通常致命的间质性肺疾病，其发病机制尚未完全明确。多项研究提供了支持和反对病毒辅助因子在特发性肺纤维化发病机制中作用的证据。在本研究中，对 Epstein-Barr 病毒（EBV）和人疱疹病毒 8 型（HHV-8）在 IPF 发病机制中的可能作用进行了研究。
采用聚合酶链反应（PCR）检测 58 份福尔马林固定石蜡包埋的肺组织标本（29 份对照标本和 29 份 IPF 标本）中的 EBV 和 HHV-8。
29 份 IPF 标本中有 6 份（20.7%）肺组织存在 EBV DNA，而 29 份对照标本中有 1 份（3.4%）存在 EBV DNA（P = 0.102）。29 例 IPF 标本中有 3 份（10.3%）检测到 HHV-8 基因。对照组未发现 HHV-8 基因证据（P = 0.227）。
尽管多项研究强烈提示 EBV 和 HHV-8 在 IPF 发生发展中起作用，但本研究中 IPF 标本与对照标本中 EBV 和 HHV-8 DNA 的患病率无统计学显著差异。
