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一种新型犬用2型灭活腺病毒疫苗对犬的免疫原性。

Immunogenicity of a new, inactivated canine adenovirus type 2 vaccine for dogs.

作者信息

Yang Dong-Kun, Kim Ha-Hyun, Yoo Jae Young, Ji Miryeon, Han Bok Hee, Oh Subin, Hyun Bang-Hun

机构信息

Viral Disease Research Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Gimcheon, Korea.

出版信息

Clin Exp Vaccine Res. 2020 Jan;9(1):40-47. doi: 10.7774/cevr.2020.9.1.40. Epub 2020 Jan 31.

DOI:10.7774/cevr.2020.9.1.40
PMID:32095439
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7024732/
Abstract

PURPOSE

We constructed a new canine adenovirus type 2 (CAV-2) vaccine candidate using the recently isolated Korean CAV-2 strain; we termed the vaccine APQA1701-40P and evaluated its safety and immunogenicity in dogs.

MATERIALS AND METHODS

To generate the anti-CAV-2 vaccine, APQA1701 was passaged 40 times in MDCK cells growing in medium containing 5 mM urea and the virus was inactivated using 0.05% (volume per volume) formaldehyde. Two vaccines were prepared by blending inactivated APQA1701-40P with two different adjuvants; both were intramuscularly injected (twice) into guinea pigs. The safety and immunogenicity of the Cabopol-adjuvanted vaccine were evaluated in seronegative dogs. The humoral responses elicited were measured using an indirect enzyme-linked immunosorbent assay (I-ELISA), and via a virus neutralization assay (VNA).

RESULTS

The new, inactivated CAV-2 vaccine strain, APQA1701-40P, lacked six amino acids of the E1b-19K protein. In guinea pigs, the Cabopol-adjuvanted vaccine afforded a slightly higher VNA titer and I-ELISA absorbance than an IMS gel-adjuvanted vaccine 4 weeks post-vaccination (p>0.05). Dogs inoculated with the former vaccine developed a significantly higher immune titer than non-vaccinated dogs.

CONCLUSION

The Cabopol-adjuvanted, inactivated CAV-2 vaccine was safe and induced a high VNA titer in dogs.

摘要

目的

我们使用最近分离出的韩国犬腺病毒2型（CAV - 2）毒株构建了一种新的候选犬用疫苗；我们将该疫苗命名为APQA1701 - 40P，并评估了其在犬类中的安全性和免疫原性。

材料与方法

为制备抗CAV - 2疫苗，将APQA1701在含有5 mM尿素的培养基中生长的MDCK细胞上传代40次，然后使用0.05%（体积/体积）甲醛对病毒进行灭活。通过将灭活的APQA1701 - 40P与两种不同的佐剂混合制备了两种疫苗；两种疫苗均通过肌肉注射（两次）接种到豚鼠体内。在血清阴性的犬类中评估了卡波姆佐剂疫苗的安全性和免疫原性。使用间接酶联免疫吸附测定（I - ELISA）和病毒中和试验（VNA）测量引发的体液免疫反应。

结果

新的灭活CAV - 2疫苗株APQA1701 - 40P缺少E1b - 19K蛋白的六个氨基酸。在豚鼠中，接种疫苗4周后，卡波姆佐剂疫苗的VNA滴度和I - ELISA吸光度略高于IMS凝胶佐剂疫苗（p>0.05）。接种前一种疫苗的犬类产生的免疫滴度明显高于未接种疫苗的犬类。

结论

卡波姆佐剂灭活CAV - 2疫苗在犬类中安全且能诱导产生高VNA滴度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/bbfd359e22a0/cevr-9-40-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/5ddc26b341f3/cevr-9-40-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/ae22c80b5cac/cevr-9-40-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/f4f0d8cc7244/cevr-9-40-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/bbfd359e22a0/cevr-9-40-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/5ddc26b341f3/cevr-9-40-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/ae22c80b5cac/cevr-9-40-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/f4f0d8cc7244/cevr-9-40-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47da/7024732/bbfd359e22a0/cevr-9-40-g004.jpg

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