Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd. South District, Taichung, Taiwan, Republic of China.
Department of Food Science & Technology, Central Taiwan University of Science and Technology, Beitun District, Taichung, Taiwan, Republic of China.
Appl Biochem Biotechnol. 2020 May;191(1):412-425. doi: 10.1007/s12010-020-03273-2. Epub 2020 Feb 25.
This study led in the pioneering technique incubated in a bioreactor with the forced air injection system. The purpose of this study was to establish the optimal incubation conditions for this technique. The results showed that the speed at which Inonotus obliquus was incubated with the forced air injection system was superior to that with a normal bioreactor. A nitrogen to oxygen ratio of 50:50 provided the best results with the forced air injection system, including in terms of the achievement of biomass, total triterpenes, betulinic acid content, and the scavenging activities of DPPH radicals, which reached up to 21.3 g/1000 mL, 2.1 g/1000 mL, 1.9 g/1000 mL, and 87.3%, respectively. The results showed that the bioreactor with the forced air injection system could more effectively incubate I. obliquus by using less vapor while still utilizing a model close to that of a traditional bioreactor. The innovative bioreactor fermentation model was thus more economical than the traditional bioreactor model.
本研究采用生物反应器强制空气注射系统孵化出开创性技术。本研究的目的是确定该技术的最佳孵化条件。结果表明，与普通生物反应器相比，采用强制空气注射系统孵化的斜卧孔菌的速度更快。强制空气注射系统的最佳氮氧比为 50:50，包括生物量、总三萜、桦木酸含量和 DPPH 自由基清除活性的实现，分别达到 21.3 g/1000 mL、2.1 g/1000 mL、1.9 g/1000 mL 和 87.3%。结果表明，强制空气注射系统的生物反应器可以在使用较少蒸汽的情况下更有效地孵化斜卧孔菌，同时仍然利用接近传统生物反应器的模型。因此，创新的生物反应器发酵模型比传统生物反应器模型更经济。
