Department of Dermatology, The People's Hospital of Danyang, Danyang, China.
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1302-1308. doi: 10.26355/eurrev_202002_20187.
Melanoma is one of the most ordinary malignant tumors. Recent studies have revealed that long noncoding RNAs (lncRNAs) play an important role in the progression of tumorigenesis. This work aims to identify how lncRNA NEAT1 functions in the progression of melanoma.
NEAT1 expression of both melanoma patients' tissue samples and cell lines was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Moreover, the function of NEAT1 was identified by performing the proliferation and transwell assay in vitro. Besides, the underlying mechanism was explored through the Luciferase assay and RNA immunoprecipitation (RIP) assay. In addition, tumor formation and metastasis assays were also conducted in vivo.
In this research, NEAT1 expression was significantly higher in melanoma tissues compared with that in skin tissues with the melanocytic nevus. Cell proliferation and invasion of melanoma were inhibited after the knockdown of NEAT1 in vitro. Moreover, the results of further experiments revealed that microRNA-224-5p (miR-224-5p) was upregulated via the knockdown of NEAT1 and was also a direct target of NEAT1 in melanoma. Furthermore, tumor formation and metastasis of melanoma were inhibited via the knockdown of NEAT1 in nude mice.
Our study suggests that NEAT1 enhances melanoma cell proliferation and metastasis via sponging miR-224-5p in vitro and in vivo.
黑色素瘤是最常见的恶性肿瘤之一。最近的研究表明，长链非编码 RNA（lncRNA）在肿瘤发生发展中起着重要作用。本研究旨在确定 lncRNA NEAT1 在黑色素瘤进展中的作用机制。
通过实时定量聚合酶链反应（RT-qPCR）检测黑色素瘤患者组织样本和细胞系中 NEAT1 的表达。此外，通过体外增殖和侵袭实验鉴定 NEAT1 的功能。通过荧光素酶报告基因检测和 RNA 免疫沉淀（RIP）实验探讨其潜在机制。此外，还进行了体内肿瘤形成和转移实验。
本研究发现，与含有黑色素细胞痣的皮肤组织相比，黑色素瘤组织中 NEAT1 的表达明显升高。体外敲低 NEAT1 后，黑色素瘤细胞的增殖和侵袭能力受到抑制。此外，进一步实验结果表明，miR-224-5p 通过敲低 NEAT1 而上调，并且是黑色素瘤中 NEAT1 的直接靶标。此外，在裸鼠体内敲低 NEAT1 可抑制黑色素瘤的形成和转移。
本研究表明，NEAT1 通过在体外和体内吸附 miR-224-5p 增强黑色素瘤细胞的增殖和转移。
