miR-135b-5p 通过靶向 RBX1 抑制恶性黑素瘤细胞的进展。
MiR-135b-5p inhibits the progression of malignant melanoma cells by targeting RBX1.
机构信息
Department of Burn and Plastic Surgery, Affiliated Success Hospital of Xiamen University (The 174th Hospital of PLA), Xiamen, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1309-1315. doi: 10.26355/eurrev_202002_20188.
OBJECTIVE
The aim of this study was to investigate the potential effects of microRNA-135b-5p (miR-135b) on the development of malignant melanoma (MM) and the relevant mechanism.
PATIENTS AND METHODS
The expression level of miR-135b in MM tissues and cells was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Online prediction software and luciferase reporter assays were used to predict and verify the possible target of miR-135b, respectively. Furthermore, the effects of the miR-135b on MM A375 cells were determined by Western blotting, MTT, and transwell assays.
RESULTS
MiR-135b was significantly down-regulated in MM. RING-box protein 1 (RBX1) was verified as a direct target of miR-135b. Subsequent experiments showed that down-regulation of RBX1 resulted from miR-135b up-regulation could significantly inhibit the proliferation, invasion, and migration abilities of MM cells.
CONCLUSIONS
MiR-135b inhibited the progression of MM by targeting RBX1. Our findings revealed that miR-135b/RBX1 might be a potential therapeutic target for the treatment of MM.
目的
本研究旨在探讨 microRNA-135b-5p（miR-135b）对恶性黑色素瘤（MM）发展的潜在影响及其相关机制。
方法
采用实时定量聚合酶链反应（qRT-PCR）检测 MM 组织和细胞中 miR-135b 的表达水平。利用在线预测软件和荧光素酶报告基因实验分别预测和验证 miR-135b 的可能靶基因。此外，通过 Western blot、MTT 和 Transwell 实验检测 miR-135b 对 MM A375 细胞的影响。
结果
miR-135b 在 MM 中显著下调。RING 盒蛋白 1（RBX1）被验证为 miR-135b 的直接靶基因。进一步的实验表明，miR-135b 上调导致 RBX1 下调可显著抑制 MM 细胞的增殖、侵袭和迁移能力。
结论
miR-135b 通过靶向 RBX1 抑制 MM 的进展。我们的研究结果表明，miR-135b/RBX1 可能成为 MM 治疗的潜在靶点。
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