Department of Plastic Surgery, Fuyang People's Hospital, Fuyang, China.
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2625-2631. doi: 10.26355/eurrev_202003_20531.
To investigate the potential effects of microRNA-429-5p (miR-429-5p) on the development of malignant melanoma (MM) and the relevant mechanism.
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the differential expression of miR-429-5p in MM tissues. The relationship between miR-429-5p expression and clinical pathological data of MM patients was analyzed. LIM kinase 1 (LIMK1) was verified as a downstream target of miR-429-5p by online prediction software, and the interaction between LIMK1 and miR-429-5p was verified by Dual-Luciferase reporter assay.
Compared with normal skin tissues, miR-429-5p was downregulated in MM tissues. MiR-429-5p expression was correlated with tumor size and stage of MM. Upregulation of miR-429-5p significantly inhibited protein expression of LIMK1 and reduced migration and invasion ability of MM cells. LIMK1 was involved in MM progression regulated by miR-429-5p.
MiR-429-5p attenuates migration and invasion in MM by targeting LIMK1. Hence, miR-429-5p/LIMK1 axis might be a potential therapeutic target for the treatment of MM.
探讨微小 RNA-429-5p（miR-429-5p）对恶性黑色素瘤（MM）发展的潜在影响及其相关机制。
采用实时定量聚合酶链反应（qRT-PCR）检测 MM 组织中 miR-429-5p 的差异表达。分析 miR-429-5p 表达与 MM 患者临床病理资料的关系。通过在线预测软件验证 LIM 激酶 1（LIMK1）是 miR-429-5p 的下游靶基因，并通过双荧光素酶报告基因检测验证 LIMK1 与 miR-429-5p 之间的相互作用。
与正常皮肤组织相比，MM 组织中 miR-429-5p 下调。miR-429-5p 的表达与 MM 的肿瘤大小和分期相关。上调 miR-429-5p 可显著抑制 LIMK1 蛋白表达，并降低 MM 细胞的迁移和侵袭能力。LIMK1 参与了 miR-429-5p 调控的 MM 进展。
miR-429-5p 通过靶向 LIMK1 减弱 MM 中的迁移和侵袭。因此，miR-429-5p/LIMK1 轴可能是 MM 治疗的潜在治疗靶点。
