Wu S-B, Wang H-Q
Department of Gastroenterology, the Second Hospital of Dalian Medical University, Dalian, China.
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1195-1201. doi: 10.26355/eurrev_202002_20171.
Esophageal squamous cell carcinoma (ESCC) is the most malignant type of esophageal cancer. Although significant advances have been made in ESCC diagnosis and therapy, its poor pathogenesis and prognosis remain a life-threatening problem. Meanwhile, long noncoding RNAs (lncRNAs) exert a pivotal function in tumorigenesis. In this research, we aimed to explore the association between the aberrant expression of lncRNA DLX6-AS1 and the development and metastasis of ESCC.
DLX6-AS1 expression was monitored by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) in ESCC specimens. Moreover, experiments were conducted to detect the effect of DLX6-AS1 on the cell proliferation and metastasis of ESCC. In addition, the underlying mechanism was further explored through luciferase assays and RNA immunoprecipitation assay (RIP).
DLX6-AS1 expression level was significantly higher in ESCC specimens. Moreover, cell proliferation and metastasis of ESCC cells could be inhibited via reducing DLX6-AS1 expression. Besides, DLX6-AS1 was regarded as an oncogene in ESCC. Furthermore, DLX6-AS1 acted as a competing endogenous RNA via sponging miR-577 in ESCC.
In summary, DLX6-AS1 promotes development and metastasis of ESCC by sponging miR-577 and could be a potential therapeutic target.
食管鳞状细胞癌（ESCC）是食管癌中恶性程度最高的类型。尽管ESCC的诊断和治疗已取得显著进展，但其不良的发病机制和预后仍然是一个危及生命的问题。同时，长链非编码RNA（lncRNA）在肿瘤发生中发挥着关键作用。在本研究中，我们旨在探讨lncRNA DLX6-AS1的异常表达与ESCC发生发展及转移之间的关联。
通过定量实时聚合酶链反应（qRT-PCR）监测ESCC标本中DLX6-AS1的表达。此外，进行实验检测DLX6-AS1对ESCC细胞增殖和转移的影响。另外，通过荧光素酶报告基因检测和RNA免疫沉淀试验（RIP）进一步探究其潜在机制。
ESCC标本中DLX6-AS1表达水平显著升高。此外，降低DLX6-AS1表达可抑制ESCC细胞的增殖和转移。此外，DLX6-AS1在ESCC中被视为癌基因。此外，在ESCC中，DLX6-AS1通过海绵吸附miR-577发挥竞争性内源RNA的作用。
综上所述，DLX6-AS1通过海绵吸附miR-577促进ESCC的发生发展及转移，可能是一个潜在的治疗靶点。
