Phase I Clinical Research Center, The Affiliated Hospital of Kangda College of Nanjing Medical University/The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China.
Department of Gastroenterology, Lianyungang Clinical College of Nanjing Medical University /The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China.
Thorac Cancer. 2020 Aug;11(8):2306-2315. doi: 10.1111/1759-7714.13551. Epub 2020 Jul 7.
Previous studies have shown that lncRNA LINC00662 plays an important role in pathogenesis of malignancies. The purpose of this study was to elucidate the regulatory mechanism of LINC00662 in esophageal squamous cell carcinoma (ESCC).
In this study, the regulatory mechanism of LINC00662 was investigated by RT-qPCR. MTT, transwell and dual luciferase reporter assays.
Upregulation of LINC00662 was found in ESCC and associated with worse clinical outcomes in ESCC patients. More importantly, knockdown of LINC00662 restrained cell proliferation, migration and invasion in ESCC. In addition, LINC00662 acts as a molecular sponge for miR-340-5p in ESCC, and miR-340-5p directly targets HOXB2. HOXB2 expression can be positively regulated by LINC00662 in ESCC. Furthermore, HOXB2 downregulation or miR-340-5p overexpression weakened the carcinogenesis of LINC00662 in ESCC.
LncRNA LINC00662 promotes the progression of ESCC by upregulating HOXB2 by sponging miR-340-5p.
先前的研究表明，长链非编码 RNA LINC00662 在恶性肿瘤的发病机制中起着重要作用。本研究旨在阐明 LINC00662 在食管鳞状细胞癌（ESCC）中的调控机制。
在这项研究中，通过 RT-qPCR、MTT、transwell 和双荧光素酶报告基因检测来研究 LINC00662 的调控机制。
LINC00662 在 ESCC 中上调，并与 ESCC 患者的更差临床结局相关。更重要的是，下调 LINC00662 可抑制 ESCC 中的细胞增殖、迁移和侵袭。此外，LINC00662 在 ESCC 中作为 miR-340-5p 的分子海绵起作用，而 miR-340-5p 可直接靶向 HOXB2。在 ESCC 中，LINC00662 可正向调节 HOXB2 的表达。此外，下调 HOXB2 或过表达 miR-340-5p 可减弱 LINC00662 在 ESCC 中的致癌作用。
LncRNA LINC00662 通过海绵吸附 miR-340-5p 来上调 HOXB2，从而促进 ESCC 的进展。
