From the Institute of Immunology, Zhongshan School of Medicine; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
From the department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
Eur Cytokine Netw. 2019 Dec 1;30(4):114-122. doi: 10.1684/ecn.2015.0368.
Tonsils are important lymphoid organs in which B cells and T cells complete their maturation and identify cells that are infected by pathogens. However, the functions of T cells in human tonsils remain unclear, especially the characteristics of polyfunctional CD4 T helper cells. In this study, we used multi-color flow cytometry to analyze the expression or co-expression of effector cytokines in CD4 T cells from tonsillar tissues. We have demonstrated that tonsillar CD4 T cell can express various Th effector cytokines after short-term polyclonal stimulation, and that cytokine-producing CD4 T cells were CD45RO T cells. In addition, we analyzed the co-expression of two or more kinds of cytokines at the level of a single cell. The results showed that tonsillar CD4 T cells exhibited polyfunctionality by co-expressing two to five kinds of cytokines in the same time. These data furnished a basic theory for further understanding the differentiation of polyfunctional Th cells in human tonsils and their functions in resisting invasive microorganisms.
扁桃体是重要的淋巴器官，其中 B 细胞和 T 细胞完成成熟并识别被病原体感染的细胞。然而，T 细胞在人扁桃体中的功能尚不清楚，特别是多功能 CD4 T 辅助细胞的特征。在这项研究中，我们使用多色流式细胞术分析了扁桃体组织中 CD4 T 细胞的效应细胞因子的表达或共表达。我们已经证明，扁桃体 CD4 T 细胞在短期多克隆刺激后可以表达各种 Th 效应细胞因子，并且产生细胞因子的 CD4 T 细胞是 CD45RO T 细胞。此外，我们还在单细胞水平上分析了两种或更多种细胞因子的共表达。结果表明，扁桃体 CD4 T 细胞通过同时共表达两种至五种细胞因子表现出多功能性。这些数据为进一步了解人扁桃体中多功能 Th 细胞的分化及其抵抗侵袭性微生物的功能提供了理论基础。
