采用流式细胞术对多克隆刺激后单细胞水平细胞因子产生的评估。
Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation.
机构信息
Maurice Müller Laboratories (Department of Biomedical Research), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland.
出版信息
Methods Mol Biol. 2021;2285:111-119. doi: 10.1007/978-1-0716-1311-5_9.
PMID:33928547
Abstract
An important hallmark for the characterisation of Th cells is their capacity for cytokine expression. In this chapter, we describe how Th cells can be restimulated polyclonally to reveal their cytokine-producing potential that can then be analysed by intracellular staining and flow cytometry.
摘要
Th 细胞的一个重要特征是其细胞因子表达能力。在本章中，我们将描述如何通过多克隆再刺激 Th 细胞来揭示其产生细胞因子的潜能，然后通过细胞内染色和流式细胞术进行分析。
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