Maurice Müller Laboratories (Department of Biomedical Research), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland.
Methods Mol Biol. 2021;2285:111-119. doi: 10.1007/978-1-0716-1311-5_9.
An important hallmark for the characterisation of Th cells is their capacity for cytokine expression. In this chapter, we describe how Th cells can be restimulated polyclonally to reveal their cytokine-producing potential that can then be analysed by intracellular staining and flow cytometry.
Th 细胞的一个重要特征是其细胞因子表达能力。在本章中，我们将描述如何通过多克隆再刺激 Th 细胞来揭示其产生细胞因子的潜能，然后通过细胞内染色和流式细胞术进行分析。
