血清白细胞介素-32 和白细胞介素-6 在肉芽肿性多血管炎和显微镜下多血管炎中的水平：与临床和生化发现的关联。

Serum levels of interleukin-32 and interleukin-6 in granulomatosis with polyangiitis and microscopic polyangiitis: association with clinical and biochemical findings.

机构信息

Medical University in Wrocław, Department and Clinic of Otolaryngology, Head and Neck Surgery, Borowska 213 Street, WrocławPoland.

Medical University in Wrocław, Department and Clinic of Nephrology and Transplantation Medicine, Borowska 213 Street, WrocławPoland.

出版信息

Eur Cytokine Netw. 2019 Dec 1;30(4):151-159. doi: 10.1684/ecn.2019.0439.

DOI:10.1684/ecn.2019.0439

PMID:32096477

Abstract

BACKGROUND

Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis is an autoimmune disorder of unknown etiology with dysregulated cytokines levels.

OBJECTIVES

The main aim of this study was to assess the clinical correlation between antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis, granulomatosis with polyangiitis (GPA) serum levels of the microscopic polyangiitis (MPA), serum levels of the proinflammatory cytokines, interleukin (IL)-32 and interleukin-6.

METHODS

Study included 71 patients, 47 with GPA and 24 with MPA. Serum IL-32 and IL-6 concentrations were analyzed in all patients, and compared with levels observed in 10 controls. IL-32 and IL-6 were evaluated using DuoSet and Quantikine HS ELISA, respectively. IL-32 and IL-6 concentrations were correlated with disease-related clinical and laboratory findings.

RESULTS

IL-32 and IL-6 levels were significantly higher in GPA and MPA than in controls, especially IL-32 levels in GPA were elevated. IL-32 concentrations correlated positively with anti-proteinase 3 - ANCA (PR3-ANCA) levels in GPA (P < 0.0001), and with anti-myeloperoxidase ANCA (MPO-ANCA) in MPA (P = 0.049). IL-32 levels correlated positively with disease activity in GPA and MPA (P < 0.0001). GPA patients with pulmonary, cutaneous, and musculoskeletal involvement presented the highest IL-6 serum levels. Cutaneous manifestations correlated positively with IL-6 levels in MPA patients (P = 0.05). ANCA-positive patients with GPA expressed significantly high IL-6 levels (P = 0.036). No significant difference in IL-32 values was observed between ANCA-positive and ANCA-negative patients.

CONCLUSIONS

Patients with GPA and MPA present higher serum IL-32 and IL-6 levels than controls. IL-32 levels correlate positively with disease activity.

摘要

背景

抗中性粒细胞胞浆抗体（ANCA）相关性血管炎是一种病因不明的自身免疫性疾病，伴有细胞因子水平失调。

目的

本研究的主要目的是评估抗中性粒细胞胞浆抗体（ANCA）相关性血管炎、肉芽肿性多血管炎（GPA）和显微镜下多血管炎（MPA）的血清水平、促炎细胞因子白细胞介素（IL）-32 和白细胞介素-6（IL-6）之间的临床相关性。

方法

研究纳入了 71 名患者，其中 47 名患有 GPA，24 名患有 MPA。对所有患者进行血清 IL-32 和 IL-6 浓度分析，并与 10 名对照者的水平进行比较。使用 DuoSet 和 Quantikine HS ELISA 分别评估 IL-32 和 IL-6。评估 IL-32 和 IL-6 浓度与疾病相关的临床和实验室发现的相关性。

结果

GPA 和 MPA 患者的 IL-32 和 IL-6 水平明显高于对照组，尤其是 GPA 患者的 IL-32 水平升高。IL-32 浓度与 GPA 中抗蛋白酶 3-ANCA（PR3-ANCA）水平呈正相关（P<0.0001），与 MPA 中抗髓过氧化物酶 ANCA（MPO-ANCA）水平呈正相关（P=0.049）。IL-32 水平与 GPA 和 MPA 中的疾病活动呈正相关（P<0.0001）。肺部、皮肤和肌肉骨骼受累的 GPA 患者血清 IL-6 水平最高。皮肤表现与 MPA 患者的 IL-6 水平呈正相关（P=0.05）。抗 PR3-ANCA 的 GPA 阳性患者表达的 IL-6 水平显著升高（P=0.036）。在抗 PR3-ANCA 的 GPA 患者中，IL-32 值无显著差异。