Department and Clinic of Otolaryngology, Head and Neck Surgery, Medical University in Wroclaw, Poland.
Department and Clinic of Urology and Urological Oncology, Medical University in Wroclaw, Poland.
Med Clin (Barc). 2021 Nov 26;157(10):464-472. doi: 10.1016/j.medcli.2020.07.036. Epub 2020 Oct 21.
Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis is a systemic, autoimmune disease. Cytokine dysregulation during active disease and clinical remission, reflects significant immunological activity in various disease stages, and might be responsible for the potential relapse of ANCA-vasculitis.
This study aimed to screen serological profiles in active granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA), and to determine their associations with clinical characteristics.
Serum IL-10, IL-12, IL-17, IL-21, IL-23, B cell activating factor (BAFF) concentrations were determined by Quantikine HS ELISA in 71 patients, 47 with GPA and 24 with MPA, and compared with 16 healthy controls. Subsequently, the correlations between serum IL-10, IL-12, IL-17, IL-21, IL-23, BAFF levels, and both laboratory and clinical abnormalities were investigated.
BAFF levels were significantly higher in GPA than MPA, and healthy controls. IL-10 and BAFF levels were elevated in GPA patients with pulmonary involvement. Higher BAFF levels might reflect severe GPA. IL-10 and IL-12 levels were higher in MPA than GPA. In MPA, IL-10 levels were highest in patients with short disease duration, and young individuals. IL-12 correlated positively with BVAS and was elevated in patients with cardiovascular involvement and nasal S. aureus carriers.
In MPA, IL-12 correlates positively with disease activity, and is significantly increased in patients with cardiovascular involvement and nasal S. aureus carriers. Increased IL-10 is observed in young MPA patients and in those with short MPA duration. Elevated BAFF and IL-10 levels are associated with pulmonary involvement in GPA. High BAFF levels might reflect severe GPA.
抗中性粒细胞胞质抗体（ANCA）相关性血管炎是一种系统性自身免疫性疾病。在活动期和临床缓解期，细胞因子失调反映了在各种疾病阶段存在显著的免疫活性，并且可能是导致 ANCA 血管炎潜在复发的原因。
本研究旨在筛选活动期肉芽肿性多血管炎（GPA）和显微镜下多血管炎（MPA）的血清学特征，并确定其与临床特征的相关性。
采用 Quantikine HS ELISA 法检测 71 例患者（47 例 GPA 和 24 例 MPA）和 16 例健康对照者的血清白细胞介素 10（IL-10）、白细胞介素 12（IL-12）、白细胞介素 17（IL-17）、白细胞介素 21（IL-21）、白细胞介素 23（IL-23）和 B 细胞激活因子（BAFF）浓度，随后分析血清 IL-10、IL-12、IL-17、IL-21、IL-23 和 BAFF 水平与实验室和临床异常的相关性。
与 MPA 和健康对照组相比，GPA 患者的 BAFF 水平显著升高。GPA 患者肺部受累者的 IL-10 和 BAFF 水平升高。较高的 BAFF 水平可能反映 GPA 较为严重。与 GPA 相比，MPA 患者的 IL-10 和 IL-12 水平更高。在 MPA 中，疾病病程较短和年轻患者的 IL-10 水平最高。IL-12 与 BVAS 呈正相关，并且在心血管受累和鼻金黄色葡萄球菌携带者的患者中升高。
在 MPA 中，IL-12 与疾病活动度呈正相关，并且在心血管受累和鼻金黄色葡萄球菌携带者的患者中显著升高。年轻的 MPA 患者和 MPA 病程较短的患者观察到升高的 IL-10。GPA 肺部受累患者的 BAFF 和 IL-10 水平升高。高 BAFF 水平可能反映 GPA 较为严重。
