Department of Hepatobiliary Surgery, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361102, China.
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China.
Biomater Sci. 2020 Mar 17;8(6):1575-1579. doi: 10.1039/c9bm02088h.
Perihilar cholangiocarcinoma (PHCC) presents a formidable challenge due to its occult anatomic location, aggressive growth, insensitivity to conventional chemotherapy, and poor prognosis. Herein, we engineered a human epidermal growth factor receptor 2 (HER2) affibody to the surface of cell membrane nanovesicles (A-NVs) in a ligand-oriented manner and loaded them with indocyanine green (ICG) as precision theranostics for PHCC treatment. The A-NVs@ICG were prepared and exhibited satisfactory targeting effects in HER2-overexpressing PHCC cells. In vivo fluorescence and photoacoustic imaging demonstrated that A-NVs@ICG promoted the accumulation of ICG in PHCC tissue, leading to enhanced tumor regression and improved anti-cancer effects when combined with photoirradiation. Therefore, bio-engineered A-NVs@ICG represent a promising nanotheranostic agent for PHCC with potential for clinical translation.
肝门部胆管癌（PHCC）由于其隐匿的解剖位置、侵袭性生长、对常规化疗不敏感和预后不良，给治疗带来了巨大挑战。在此，我们通过配体导向的方式将人表皮生长因子受体 2（HER2）亲和体工程化到细胞膜纳米囊泡（A-NVs）的表面，并将其装载吲哚菁绿（ICG）作为 PHCC 治疗的精准治疗药物。制备了 A-NVs@ICG，并在过表达 HER2 的 PHCC 细胞中表现出令人满意的靶向效果。体内荧光和光声成像表明，A-NVs@ICG 促进了 ICG 在 PHCC 组织中的积累，当与光辐射结合时，导致肿瘤的消退增强和抗癌效果的改善。因此，生物工程化的 A-NVs@ICG 代表了一种有前途的 PHCC 纳米治疗药物，具有临床转化的潜力。
