高效液相色谱法测定血浆中拉贝洛尔时荧光检测的优化
Optimization of fluorescence detection for the determination of labetalol in plasma by high-performance liquid chromatography.
作者信息
Ostrovská V, Svobodová X, Pechová A, Kusala S, Svoboda M
机构信息
Drug Research Institute, Modra, Czechoslovakia.
出版信息
J Chromatogr. 1988 Jul 27;446:323-7. doi: 10.1016/s0021-9673(00)94448-0.
PMID:3209660
Abstract
A sensitive reversed-phase high-performance liquid chromatographic method for the determination of labetalol has been developed. A mobile phase consisting of citrate buffer (pH 6.5), acetonitrile and 2-propanol and an RP-8 column were used. The sensitivity of the fluorescence detection was enhanced to 1 ng/ml of labetalol in plasma by optimizing the emitted light. General guidelines for optimization of fluorescence detection are discussed.
摘要
已开发出一种用于测定拉贝洛尔的灵敏反相高效液相色谱法。使用了由柠檬酸盐缓冲液（pH 6.5）、乙腈和2-丙醇组成的流动相以及RP-8柱。通过优化发射光，荧光检测的灵敏度提高到血浆中拉贝洛尔1 ng/ml。讨论了荧光检测优化的一般指南。
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