无需衍生化即可测定血浆样品中拉贝洛尔非对映异构体的灵敏高效液相色谱法。

Sensitive high-performance liquid chromatographic method for the determination of labetalol diastereoisomers in plasma samples without derivatization.

作者信息

Grellet J, Michel-Gueroult P, Ducint D, Saux M C

机构信息

Pharmacokinetic and Clinical Pharmacy Laboratory, Haut-Lévèque Hospital and University of Bordeaux II, Pessac, France.

出版信息

J Chromatogr. 1994 Jan 14;652(1):59-66. doi: 10.1016/0378-4347(93)e0374-y.

DOI:10.1016/0378-4347(93)e0374-y

PMID:8014228

Abstract

A direct high-performance liquid chromatographic assay for the determination of labetalol diastereoisomers in plasma without derivatization was developed. Baseline resolution of diastereoisomers was accomplished on a C18 bonded reversed-phase polymeric column with a basic (pH 11.5) mobile phase and isocratic elution. Sample treatment was optimized in order to achieve a complete extraction of labetalol diastereoisomers and to avoid racemization during extraction. Fluorimetric detection improved the selectivity and afforded a detection limit of 3 ng/ml for each diastereoisomer. This method is suitable for routine quantification of labetalol diastereoisomers and has been applied to a pharmacokinetic study in small laboratory animals.

摘要

建立了一种直接高效液相色谱法，用于不经衍生化测定血浆中的拉贝洛尔非对映异构体。在C18键合反相聚合物柱上，采用碱性（pH 11.5）流动相和等度洗脱实现了非对映异构体的基线分离。对样品处理进行了优化，以实现拉贝洛尔非对映异构体的完全萃取，并避免萃取过程中的消旋化。荧光检测提高了选择性，每种非对映异构体的检测限为3 ng/ml。该方法适用于拉贝洛尔非对映异构体的常规定量分析，并已应用于小型实验动物的药代动力学研究。