Xue W L, Carlson R M
Department of Chemistry, University of Minnesota, Duluth 55812.
J Chromatogr. 1988 Aug 5;447(1):81-90. doi: 10.1016/0021-9673(88)90008-8.
The retention behavior of thirteen alkylated guanines on normal-phase silica gel and amino columns and on reversed-phase ODS and phenyl columns was studied. The larger the alkyl substituent at the same position of guanine the weaker was the retention in the normal-phase chromatographic system and the greater the retention during reversed-phase chromatography. O6-Derivatives possess the lowest polarity in each set of isomers. An amino column was found to be of highest efficiency in terms of separation of the set of ethylguanine isomers and of benzylguanines studied. A phenyl column provided the best resolution of methylated guanines.
研究了13种烷基化鸟嘌呤在正相硅胶柱、氨基柱以及反相ODS柱和苯基柱上的保留行为。在鸟嘌呤相同位置上的烷基取代基越大，在正相色谱系统中的保留越弱，而在反相色谱中的保留越强。在每组异构体中，O6 - 衍生物的极性最低。对于所研究的乙基鸟嘌呤异构体和苄基鸟嘌呤，发现氨基柱在分离方面效率最高。苯基柱对甲基化鸟嘌呤提供了最佳的分离度。
