Du Ling, Wang Lina, Yu Tong, Xin Ruolei, Meng Zhefeng
Minhang Hospital, Fudan University, No.170, Xingsong Road, Minhang District, Shanghai, China.
The Third People's Hospital of Zhenjiang City, Jiangsu Province, Zhenjiang, China.
Curr HIV Res. 2020;18(3):165-171. doi: 10.2174/1570162X18666200225113857.
HIV-1 CRF01_AE and CRF07_BC recombinant strains are responsible for more than 80% of new infections in China since the beginning of the 2000s. These two strains may have distinct genetic mutations, which resulted in distinct patterns of pathogenesis related to the viral gene, Vpr.
The amino acid pattern and genetic diversity of Vpr were analyzed and characterized in HIV-1 CRF01_AE and CRF07_BC HIV-1 strains.
The Vpr gene was amplified from extracted viral RNA and DNA sequencing was performed using an ABI3730 analyzer. The positional amino acid composition, genetic variation and distance of Vpr sequence were analyzed by Bio-Edit 7.2 and Mega 6.01 software packages.
A total of 162 CRF01_AE and 80 CRF07_BC derived Vpr sequences were obtained by DNA sequencing. CRF01_AE patients showed higher viral load and lower CD4 counts than CRF07_BC patients (P<0.05). Higher genetic distance and more polymorphic amino acids were found in CRF01_AE Vpr than CRF07_BC Vpr (P<0.05). The common conservative amino acid region was identified as 29EAVRHFP35 in both CRF07_BC and CRF01_AE. Of note, the R77Q mutation was found in both the most recently Chinese derived CRF07_BC and CRF01_AE Vpr.
CRF01_AE derived Vpr has higher genetic variation and pathogenesis in comparison to the CRF07_BC strain.
自21世纪初以来，HIV-1 CRF01_AE和CRF07_BC重组毒株导致了中国80%以上的新感染病例。这两种毒株可能存在不同的基因突变，从而导致与病毒基因Vpr相关的不同发病机制模式。
分析并鉴定HIV-1 CRF01_AE和CRF07_BC HIV-1毒株中Vpr的氨基酸模式和遗传多样性。
从提取的病毒RNA中扩增Vpr基因，并使用ABI3730分析仪进行DNA测序。通过Bio-Edit 7.2和Mega 6.01软件包分析Vpr序列的位置氨基酸组成、遗传变异和距离。
通过DNA测序共获得162个CRF01_AE和80个CRF07_BC来源的Vpr序列。CRF01_AE患者的病毒载量高于CRF07_BC患者，CD4计数低于CRF07_BC患者（P<0.05）。与CRF07_BC Vpr相比，CRF01_AE Vpr具有更高的遗传距离和更多的多态性氨基酸（P<0.05）。在CRF07_BC和CRF01_AE中，共同的保守氨基酸区域均被鉴定为29EAVRHFP35。值得注意的是，在最新的中国来源的CRF07_BC和CRF01_AE Vpr中均发现了R77Q突变。
与CRF07_BC毒株相比，CRF01_AE来源的Vpr具有更高的遗传变异和发病机制。
