伴随精原干细胞分化的代谢变化。

Metabolic Changes Accompanying Spermatogonial Stem Cell Differentiation.

机构信息

Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, the University of Newcastle, Callaghan, Newcastle, NSW 2300, Australia; Hunter Medical Research Institute, Pregnancy and Reproduction Program, New Lambton Heights, Newcastle, NSW 2305, Australia.

出版信息

Dev Cell. 2020 Feb 24;52(4):399-411. doi: 10.1016/j.devcel.2020.01.014.

DOI:10.1016/j.devcel.2020.01.014

PMID:32097651

Abstract

Male fertility is driven by spermatogonial stem cells (SSCs) that self-renew while also giving rise to differentiating spermatogonia. Spermatogonial transitions are accompanied by a shift in gene expression, however, whether equivalent changes in metabolism occur remains unexplored. In this review, we mined recently published scRNA-seq databases from mouse and human testes to compare expression profiles of spermatogonial subsets, focusing on metabolism. Comparisons revealed a conserved upregulation of genes involved in mitochondrial function, biogenesis, and oxidative phosphorylation in differentiating spermatogonia, while gene expression in SSCs reflected a glycolytic cell. Here, we also discuss the relationship between metabolism and the external microenvironment within which spermatogonia reside.

摘要

男性生育能力由精原干细胞（SSC）驱动，SSC 既能自我更新，又能产生分化的精原细胞。精原细胞的转变伴随着基因表达的转变，然而，代谢是否发生等效变化仍未被探索。在这篇综述中，我们挖掘了最近发表的来自小鼠和人类睾丸的 scRNA-seq 数据库，以比较精原细胞亚群的表达谱，重点关注代谢。比较发现，分化的精原细胞中线粒体功能、生物发生和氧化磷酸化相关基因的表达上调，而 SSCs 中的基因表达则反映了一个糖酵解细胞。在这里，我们还讨论了代谢与精原细胞所处的外部微环境之间的关系。