Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, the University of Newcastle, Callaghan, Newcastle, NSW 2300, Australia; Hunter Medical Research Institute, Pregnancy and Reproduction Program, New Lambton Heights, Newcastle, NSW 2305, Australia.
Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, the University of Newcastle, Callaghan, Newcastle, NSW 2300, Australia; Hunter Medical Research Institute, Pregnancy and Reproduction Program, New Lambton Heights, Newcastle, NSW 2305, Australia.
Dev Cell. 2020 Feb 24;52(4):399-411. doi: 10.1016/j.devcel.2020.01.014.
Male fertility is driven by spermatogonial stem cells (SSCs) that self-renew while also giving rise to differentiating spermatogonia. Spermatogonial transitions are accompanied by a shift in gene expression, however, whether equivalent changes in metabolism occur remains unexplored. In this review, we mined recently published scRNA-seq databases from mouse and human testes to compare expression profiles of spermatogonial subsets, focusing on metabolism. Comparisons revealed a conserved upregulation of genes involved in mitochondrial function, biogenesis, and oxidative phosphorylation in differentiating spermatogonia, while gene expression in SSCs reflected a glycolytic cell. Here, we also discuss the relationship between metabolism and the external microenvironment within which spermatogonia reside.
男性生育能力由精原干细胞（SSC）驱动，SSC 既能自我更新，又能产生分化的精原细胞。精原细胞的转变伴随着基因表达的转变，然而，代谢是否发生等效变化仍未被探索。在这篇综述中，我们挖掘了最近发表的来自小鼠和人类睾丸的 scRNA-seq 数据库，以比较精原细胞亚群的表达谱，重点关注代谢。比较发现，分化的精原细胞中线粒体功能、生物发生和氧化磷酸化相关基因的表达上调，而 SSCs 中的基因表达则反映了一个糖酵解细胞。在这里，我们还讨论了代谢与精原细胞所处的外部微环境之间的关系。
