Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P, India.
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P, India.
Toxicol In Vitro. 2020 Jun;65:104810. doi: 10.1016/j.tiv.2020.104810. Epub 2020 Feb 22.
Bioallethrin is an insecticide that is widely used to control mosquitoes, fleas and cockroaches. The widespread use of bioallethrin has resulted in both occupational and non-occupational human exposure. Bioallethrin enters blood, regardless of the route of exposure, where it can interact with erythrocytes. We have studied the effect of bioallethrin on isolated human erythrocytes under in vitro conditions. Erythrocytes were incubated with increasing concentrations of bioallethrin (10-200 μM) for 4 h at 37 °C. Several biochemical parameters were analyzed in bioallethrin treated and untreated (control) cells. Incubation of erythrocytes with bioallethrin increased protein oxidation, lipid peroxidation and depleted sulfhydryl group content. Membrane damage was evident from cell lysis, osmotic fragility, inhibition of bound enzymes and transmembrane electron transport system. Bioallethrin also increased hemoglobin oxidation, heme degradation and the release of free iron moiety. This will decrease the oxygen transporting ability of blood. Bioallethrin treatment altered the specific activities of antioxidant enzymes and diminished the antioxidant power of cells. Scanning electron microscopy showed that bioallethrin treatment also altered erythrocyte mophology. Almost all changes were in a bioallethrin concentration dependent manner. The cytotoxicity of bioallethrin is probably mediated by reactive oxygen and nitrogen species whose formation was significantly enhanced in treated erythrocytes. Thus bioallethrin enhances the generation of reactive species which cause oxidative damage of cell components in human erythrocytes.
生物丙烯菊酯是一种杀虫剂，广泛用于控制蚊子、跳蚤和蟑螂。生物丙烯菊酯的广泛使用导致了职业和非职业人群的人类暴露。生物丙烯菊酯进入血液，无论暴露途径如何，都可以与红细胞相互作用。我们已经在体外条件下研究了生物丙烯菊酯对分离的人红细胞的影响。将红细胞在 37°C 下用递增浓度的生物丙烯菊酯（10-200μM）孵育 4 小时。在生物丙烯菊酯处理和未处理（对照）的细胞中分析了几种生化参数。用生物丙烯菊酯孵育红细胞会增加蛋白质氧化、脂质过氧化和巯基含量的消耗。细胞膜损伤可从细胞溶解、渗透脆性、结合酶和跨膜电子传递系统的抑制中看出。生物丙烯菊酯还增加了血红蛋白氧化、血红素降解和游离铁部分的释放。这将降低血液的携氧能力。生物丙烯菊酯处理改变了抗氧化酶的特异性活性，并降低了细胞的抗氧化能力。扫描电子显微镜显示，生物丙烯菊酯处理还改变了红细胞的形态。几乎所有变化都与生物丙烯菊酯浓度呈依赖性。生物丙烯菊酯的细胞毒性可能是由活性氧和氮物种介导的，这些物质在处理过的红细胞中的形成显著增强。因此，生物丙烯菊酯增强了活性物质的生成，导致人红细胞中细胞成分的氧化损伤。
