Department of Pharmacy, Health and Nutritional Sciences, Department of Excellence 2018-2022, University of Calabria, Edificio Polifunzionale, 87036 Rende (CS), Italy.
Department of Chemistry and Chemical Technologies, University of Calabria, 87036 Rende (CS), Italy.
Molecules. 2020 Feb 21;25(4):968. doi: 10.3390/molecules25040968.
Glucans possess a broad spectrum of biological activities. In this context, the present study was performed to isolate glucans from an Italian cultivar of Mill. at three different harvesting periods, in order to evaluate their effects on wound healing. The dry fruits were subjected to an alkaline extraction and then isolated glucans were purified by dialyzation. The crude and soluble samples were characterized by FT-IR and SEM analyses. Afterwards, total, α- and β-glucan content was measured using an enzymatic procedure. The results highlighted that the glucan amount increased as the maturation proceeded as well as the β-glucan percentage, which ranged from 48.2 at the first harvesting to 65.4 at the third harvesting. Furthermore, the effects of isolated glucans on the viability and migration of keratinocytes were evaluated using the in vitro MTT and scratch wound assays. The best proliferative effects on keratinocyte migration have been achieved with soluble glucans from third harvesting at 100 μM after 24 and 48 h (*** < 0.001). The same treated group showed significant narrowing of the scratch area after 24 h and complete closure of the injury after 48 h. The findings highlighted the effectiveness of soluble glucans on regeneration of damaged skin.
葡聚糖具有广泛的生物活性。在这种情况下，本研究从意大利栽培的 Mill. 中分离葡聚糖进行了研究，在三个不同的收获期进行，以评估它们对伤口愈合的影响。将干果实进行碱性提取，然后通过透析分离纯化葡聚糖。采用 FT-IR 和 SEM 分析对粗提物和可溶性样品进行了表征。之后，采用酶法测定总、α-和β-葡聚糖含量。结果表明，随着成熟度的增加，葡聚糖的含量以及β-葡聚糖的百分比也增加，从第一次收获时的 48.2%增加到第三次收获时的 65.4%。此外，还通过体外 MTT 和划痕伤口试验评估了分离的葡聚糖对角质形成细胞活力和迁移的影响。在 24 和 48 小时时，用浓度为 100 μM 的第三次收获的可溶性葡聚糖处理，对角质形成细胞迁移的增殖作用最佳（*** < 0.001）。经相同处理的组在 24 小时后划痕面积明显变窄，48 小时后伤口完全愈合。研究结果强调了可溶性葡聚糖在受损皮肤再生方面的有效性。
