Sakaguchi Camila A, Nieman David C, F Signini Etore, M de Abreu Raphael, Silva Claudio D, Rehder-Santos Patrícia, Carosio Maria G A, M Maria Roberta, Dato Carla C, de Araújo Heloisa S S, Venâncio Tiago, Ferreira Antônio G, Catai Aparecida M
Physical Therapy Department, Federal University of São Carlos, São Carlos, SP 13565-905, Brazil.
North Carolina Research Campus, Appalachian State University, Kannapolis, NC 28081, USA.
Metabolites. 2020 Feb 21;10(2):78. doi: 10.3390/metabo10020078.
This study investigated the chronic effect of inspiratory muscle training (IMT) on the human serum metabolome in healthy male recreational cyclists. Using a randomized, parallel group design, twenty-eight participants were randomized to three IMT groups: low intensity (LI, = 7); moderate intensity (MI, = 10); and high intensity (HI, = 11). The IMT was performed for 11 weeks. Another group of participants under the same conditions, who did not perform the IMT but participated in all procedures, was included as controls (CG, = 6). Blood samples were collected one week before and after 11 weeks of IMT and analyzed for metabolite shifts using 1H NMR. Statistical analysis included a 4 (group) × 2 (time) repeated measures ANOVA using the general linear model (GLM), and multivariate principal component analysis (PCA). Untargeted metabolomics analysis of serum samples identified 22 metabolites, including amino acids, lipids, and tricarboxylic acid cycle intermediates. Metabolites shifts did not differ between groups, indicating that IMT at three intensity levels did not alter the serum metabolome relative to the control group. These results reveal novel insights into the metabolic effects of the IMT and are consistent with the results from other studies showing negligible chronic alterations in the serum metabolome in response to physical training.
本研究调查了吸气肌训练（IMT）对健康男性业余自行车运动员血清代谢组的长期影响。采用随机平行组设计，28名参与者被随机分为三个IMT组：低强度组（LI，n = 7）；中等强度组（MI，n = 10）；高强度组（HI，n = 11）。IMT进行了11周。另一组在相同条件下未进行IMT但参与了所有程序的参与者作为对照组（CG，n = 6）。在IMT进行11周前后各采集一次血样，并使用1H NMR分析代谢物变化。统计分析包括使用通用线性模型（GLM）的4（组）×2（时间）重复测量方差分析，以及多变量主成分分析（PCA）。血清样本的非靶向代谢组学分析鉴定出22种代谢物，包括氨基酸、脂质和三羧酸循环中间体。各组之间的代谢物变化没有差异，表明三个强度水平的IMT相对于对照组并未改变血清代谢组。这些结果揭示了关于IMT代谢效应的新见解，并且与其他研究结果一致，即体育锻炼对血清代谢组的长期改变可忽略不计。
