ICGM, Univ Montpellier, CNRS, case 1701, Place Eugène Bataillon, CEDEX 05, 34095 Montpellier, France.
Departament de Química and Centro de Innovación en Química Avanzada (ORFEO-CINQA), Facultat de Ciències, Universitat Autònoma de Barcelona, UAB Campus, C/dels Til.lers, 08193 Cerdanyola del Vallès (Barcelona), Spain.
Molecules. 2020 Feb 21;25(4):974. doi: 10.3390/molecules25040974.
Periodic Mesoporous Organosilica Nanoparticles (PMONPs) are nanoparticles of high interest for nanomedicine applications. These nanoparticles are not composed of silica (SiO). They belong to hybrid organic-inorganic systems. We considered using these nanoparticles for CO release as a contrast agent for High Intensity Focused Ultrasounds (HIFU). Three molecules (-) possessing two to four triethoxysilyl groups were synthesized through click chemistry. These molecules possess a tert-butoxycarbonyl (BOC) group whose cleavage in water at 90-100 °C releases CO. Bis(triethoxysilyl)ethylene was mixed with the molecules (or not for ) at a proportion of 90/10 to 75/25, and the polymerization triggered by the sol-gel procedure led to PMONPs. PMONPs were characterized by different techniques, and nanorods of 200-300 nm were obtained. These nanorods were porous at a proportion of 90/10, but non-porous at 75/25. Alternatively, molecules alone led to mesoporous nanoparticles of 100 nm diameter. The BOC group was stable, but it was cleaved at pH 1 in boiling water. Molecules possessing a BOC group were successfully used for the preparation of nanoparticles for CO release. The BOC group was stable and we did not observe release of CO under HIFU at lysosomal pH of 5.5. The pH needed to be adjusted to 1 in boiling water to cleave the BOC group. Nevertheless, the concept is interesting for HIFU theranostic agents.
周期性介孔有机硅纳米颗粒（PMONPs）是一种在纳米医学应用中具有高关注度的纳米颗粒。这些纳米颗粒不是由二氧化硅（SiO）组成的。它们属于混合有机-无机系统。我们考虑将这些纳米颗粒用于 CO 释放，作为高强度聚焦超声（HIFU）的对比剂。通过点击化学合成了三个具有两个到四个三乙氧基硅基的分子（-）。这些分子具有叔丁氧基羰基（BOC）基团，在 90-100°C 的水中裂解会释放 CO。将双（三乙氧基硅基）乙烯与分子（或不与分子混合）以 90/10 到 75/25 的比例混合，通过溶胶-凝胶过程引发聚合反应生成 PMONPs。PMONPs 通过不同的技术进行了表征，得到了 200-300nm 的纳米棒。这些纳米棒在 90/10 的比例下是多孔的，但在 75/25 的比例下是无孔的。或者，单独的分子导致 100nm 直径的介孔纳米颗粒。BOC 基团是稳定的，但在沸水的 pH1 下会裂解。具有 BOC 基团的分子成功地用于 CO 释放纳米颗粒的制备。BOC 基团是稳定的，我们在溶酶体 pH 值为 5.5 的 HIFU 下没有观察到 CO 的释放。需要将 pH 值调节到 1，在沸水中裂解 BOC 基团。然而，对于 HIFU 治疗学试剂来说，这一概念是有趣的。
