使用明胶和支链淀粉对活细胞进行静电纺丝。

Nosoudi Nasim, Oommen Anson Jacob, Stultz Savannah, Jordan Micah, Aldabel Seba, Hohne Chandra, Mosser James, Archacki Bailey, Turner Alliah, Turner Paul

Department of biomedical engineering, College of Engineering and Computer Sciences (CECS), Marshall University, Weisberg Family Applied Engineering Complex, Huntington, WV 25755, USA.

Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA.

Bioengineering (Basel). 2020 Feb 22;7(1):21. doi: 10.3390/bioengineering7010021.

Electrospinning is a scaffold production method that utilizes electric force to draw a polymer solution into nanometer-sized fibers. By optimizing the polymer and electrospinning parameters, a scaffold is created with the desired thickness, alignment, and pore size. Traditionally, cells and biological constitutes are implanted into the matrix of the three-dimensional scaffold following electrospinning. Our design simultaneously introduces cells into the scaffold during the electrospinning process at 8 kV. In this study, we achieved 90% viability of adipose tissue-derived stem cells through electrospinning.

静电纺丝是一种支架生产方法，它利用电力将聚合物溶液拉伸成纳米级纤维。通过优化聚合物和静电纺丝参数，可以制造出具有所需厚度、排列和孔径的支架。传统上，在静电纺丝后将细胞和生物成分植入三维支架的基质中。我们的设计在8 kV的静电纺丝过程中同时将细胞引入支架。在本研究中，我们通过静电纺丝实现了脂肪组织来源干细胞90%的存活率。