Habib Noman, Ali Qasim, Ali Shafaqat, Javed Muhammad Tariq, Zulqurnain Haider Muhammad, Perveen Rashida, Shahid Muhammad Rizwan, Rizwan Muhammad, Abdel-Daim Mohamed M, Elkelish Amr, Bin-Jumah May
Department of Botany, Government College University, Faisalabad 38000, Pakistan.
Department of Environmental Sciences and Engineering, Government College University, Faisalabad 38000, Pakistan.
Plants (Basel). 2020 Feb 22;9(2):285. doi: 10.3390/plants9020285.
The present experiment was carried out to study the influences of exogenously-applied nitric oxide (NO) donor sodium nitroprusside (SNP) and hydrogen peroxide (HO) as seed primers on growth and yield in relation with different physio-biochemical parameters, antioxidant activities, and osmolyte accumulation in wheat plants grown under control (100% field capacity) and water stress (60% field capacity) conditions. During soaking, the seeds were covered and kept in completely dark. Drought stress markedly reduced the plant growth, grain yield, leaf photosynthetic pigments, total phenolic content (TPC), total soluble proteins (TSP), leaf water potential (Ψ), leaf turgor potential (Ψ), osmotic potential (Ψ), and leaf relative water content (LRWC), while it increased the activities of enzymatic antioxidants and the accumulation of leaf ascorbic acid (AsA), proline (Pro), glycine betaine (GB), malondialdehyde (MDA), and HO. However, seed priming with SNP and HO alone and in combination mitigated the deleterious effects of water stress on growth and yield by improving the Ψ, Ψ, Ψ, photosynthetic pigments, osmolytes accumulation (GB and Pro), TSP, and the antioxidative defense mechanism. Furthermore, the application of NO and HO as seed primers also reduced the accumulation of HO and MDA contents. The effectiveness was treatment-specific and the combined application was also found to be effective. The results revealed that exogenous application of NO and HO was effective in increasing the tolerance of wheat plants under drought stress in terms of growth and grain yield by regulating plant-water relations, the antioxidative defense mechanism, and accumulation of osmolytes, and by reducing the membrane lipid peroxidation.
本实验旨在研究外源施用一氧化氮（NO）供体硝普钠（SNP）和过氧化氢（HO）作为种子引发剂，对在对照（100%田间持水量）和水分胁迫（60%田间持水量）条件下生长的小麦植株的生长和产量的影响，以及与不同生理生化参数、抗氧化活性和渗透调节物质积累的关系。浸种期间，种子被覆盖并置于完全黑暗中。干旱胁迫显著降低了植株生长、籽粒产量、叶片光合色素、总酚含量（TPC）、总可溶性蛋白（TSP）、叶片水势（Ψ）、叶片膨压势（Ψ）、渗透势（Ψ）和叶片相对含水量（LRWC），同时增加了酶促抗氧化剂的活性以及叶片抗坏血酸（AsA）、脯氨酸（Pro）、甘氨酸甜菜碱（GB）、丙二醛（MDA）和HO的积累。然而，单独和组合使用SNP和HO进行种子引发，通过改善Ψ、Ψ、Ψ、光合色素、渗透调节物质积累（GB和Pro）、TSP以及抗氧化防御机制，减轻了水分胁迫对生长和产量的有害影响。此外，将NO和HO作为种子引发剂施用还减少了HO和MDA含量的积累。效果具有处理特异性，联合施用也被证明是有效的。结果表明，外源施用NO和HO通过调节植物水分关系、抗氧化防御机制和渗透调节物质的积累，并减少膜脂过氧化，有效地提高了干旱胁迫下小麦植株的生长和籽粒产量耐受性。
