Cristea Gabriela, Feher Ioana, Voica Cezara, Radu Stelian, Magdas Dana Alina
Department of Mass Spectrometry, Chromatography and Ion Physics, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania.
Isotopes Environ Health Stud. 2020 Mar;56(1):69-82. doi: 10.1080/10256016.2020.1720672. Epub 2020 Feb 25.
In this study, three chemometric models for vegetables growing system (field versus greenhouse), geographical origin and species attribution using stable isotope (δC, δO, δH) and elemental fingerprints of 101 samples (54 squashes and 47 radishes) commercialized on Romanian market were developed. These models were constructed and validated through linear discriminant analysis. Initial validations of 94.4% and 83% were obtained for squash and radish growing systems, respectively, such that one squash and four radish samples declared to be grown in the field were attributed to the greenhouse group. For this purpose, the most powerful differentiation markers appeared to be Sn and δC for radishes, and Sn, Cu for squashes. Regarding the vegetable origin, four samples, initially considered to originate from Romania (95% for initial classification) were attributed to the foreign group in the cross-validation procedure (93.1%). Romanian radishes and squashes were characterized by a higher content of Na and Cu, respectively, compared with foreign samples, while the mean values for Zn, Sr, Zr and Co concentrations were found to be higher for the vegetables from abroad.
在本研究中，利用罗马尼亚市场上101份商品化样品（54份南瓜和47份萝卜）的稳定同位素（δC、δO、δH）和元素指纹图谱，开发了三种用于蔬菜种植系统（田间种植与温室种植）、地理来源和品种归属的化学计量学模型。这些模型通过线性判别分析构建和验证。南瓜和萝卜种植系统的初始验证准确率分别为94.4%和83%，即有1份宣称在田间种植的南瓜样品和4份宣称在田间种植的萝卜样品被归为温室种植组。为此，最具区分力的标记物对于萝卜而言似乎是Sn和δC，对于南瓜而言是Sn和Cu。关于蔬菜产地，在交叉验证过程中，4份最初被认为原产于罗马尼亚（初始分类准确率为95%）的样品被归为国外组（准确率为93.1%）。与国外样品相比，罗马尼亚萝卜和南瓜的特征分别是Na和Cu含量较高，而国外蔬菜的Zn、Sr、Zr和Co浓度平均值较高。
