Clinic of Infectious Diseases, Department of Medicine, University of Perugia, P.le Gambuli 1, 06132, Perugia, Italy.
Medical Microbiology, Department of Medicine, University of Perugia, P.le Gambuli 1, 06132, Perugia, Italy.
Diagn Microbiol Infect Dis. 2020 May;97(1):115002. doi: 10.1016/j.diagmicrobio.2020.115002. Epub 2020 Jan 29.
Detection of etiological agents is pivotal for adequate therapy of osteoarticular bacterial infections. Culture often lacks sensitivity, especially in patients under antibiotic therapy. The present study investigates the potential clinical utility of the commercial multiplex real-time polymerase chain reaction SeptiFast® (SF) in the etiological diagnosis of osteoarticular infections. Results obtained from conventional culture and SF were compared in 86 osteoarticular specimens collected from patients with suspected infection. The number of specimens positive by SF (38/86, 44.18%) was significantly greater (P = 0.001) than that of specimens positive by culture (20/86, 23.25%). The sensitivity of SF was 48.71%, significantly higher than culture sensitivity (25.64%). Specificity was 100% for both tests. The overall diagnostic accuracy for SF was 53.48%, and that of culture was 32.55%. Even with the limitation of the low number of specimens, this study supports the usefulness of SF in the diagnosis of osteoarticular infections.
病因检测对于骨和关节细菌感染的充分治疗至关重要。培养法通常缺乏敏感性，特别是在接受抗生素治疗的患者中。本研究探讨了商业多重实时聚合酶链反应 SeptiFast®（SF）在骨和关节感染病因诊断中的潜在临床应用价值。对 86 例疑似感染患者的骨和关节标本进行了常规培养和 SF 检测，比较了两种方法的检测结果。SF 阳性的标本数量（38/86，44.18%）明显多于培养阳性的标本数量（20/86，23.25%）（P = 0.001）。SF 的敏感性为 48.71%，显著高于培养法的敏感性（25.64%）。两种方法的特异性均为 100%。SF 的总体诊断准确性为 53.48%，培养法的诊断准确性为 32.55%。即使考虑到标本数量较少的局限性，本研究仍支持 SF 在骨和关节感染诊断中的应用价值。
