Li Cong, Wang Xinhao, Song Qing
Department of Surgery, General Hospital of Chinese PLA, Beijing 100853, People's Republic of China.
Department of Surgery Intensive Care Unit, General Hospital of Chinese PLA, Beijing 100853, People's Republic of China.
Onco Targets Ther. 2020 Jan 31;13:981-988. doi: 10.2147/OTT.S228576. eCollection 2020.
Hepatocellular carcinoma (HCC) is the third major cause of cancer-related death. Mounting evidence shows that microRNAs play critical roles in the initiation and progression of HCC and may potentially serve as diagnostic markers for HCC.
In the present study, we explored the biological effects of miR-885-5p on HCC progression. We performed flow cytometry analyses of miR-885-5p in HCC cell lines and identified miR-885-5p as a recurrence-related microRNA. Overexpression of miR-885-5p significantly inhibited cell migration, invasion, proliferation, angiogenesis and EMT. Then, the correlation of miR-885-5p and AEG1 were confirmed by using luciferase assays, quantitative real-time PCR analysis and Western blotting. It was subsequently confirmed that Astrocyte Elevated Gene1 (AEG1) was a direct target gene of miR-885-5p.
miR-885-5p likely acts as a tumor suppressor by regulating AEG1, suggesting that miR-885-5p may be a potential biomarker and can be targeted in therapeutic strategies against HCC in the future.
肝细胞癌（HCC）是癌症相关死亡的第三大主要原因。越来越多的证据表明，微小RNA在HCC的发生和发展中起关键作用，并可能潜在地作为HCC的诊断标志物。
在本研究中，我们探讨了miR-885-5p对HCC进展的生物学影响。我们对HCC细胞系中的miR-885-5p进行了流式细胞术分析，并将miR-885-5p鉴定为与复发相关的微小RNA。miR-885-5p的过表达显著抑制细胞迁移、侵袭、增殖、血管生成和上皮-间质转化。然后，通过荧光素酶报告基因检测、定量实时PCR分析和蛋白质印迹法证实了miR-885-5p与AEG1的相关性。随后证实星形胶质细胞上调基因1（AEG1）是miR-885-5p的直接靶基因。
miR-885-5p可能通过调节AEG1发挥肿瘤抑制作用，这表明miR-885-5p可能是一种潜在的生物标志物，并且在未来针对HCC的治疗策略中可以作为靶点。
